Protein Info for BPHYT_RS33775 in Burkholderia phytofirmans PsJN

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1154 TIGR02456: trehalose synthase" amino acids 42 to 578 (537 residues), 927.5 bits, see alignment E=2.2e-283 PF00128: Alpha-amylase" amino acids 50 to 495 (446 residues), 307.8 bits, see alignment E=2.6e-95 PF16657: Malt_amylase_C" amino acids 497 to 575 (79 residues), 86.8 bits, see alignment 1.3e-28 TIGR02457: putative maltokinase" amino acids 595 to 1120 (526 residues), 621.2 bits, see alignment E=1.6e-190 PF18085: Mak_N_cap" amino acids 626 to 680 (55 residues), 22.5 bits, see alignment 2.8e-08

Best Hits

KEGG orthology group: K05343, maltose alpha-D-glucosyltransferase [EC: 5.4.99.16] (inferred from 71% identity to bac:BamMC406_6321)

Predicted SEED Role

"Trehalose synthase (EC 5.4.99.16)" in subsystem Trehalose Biosynthesis (EC 5.4.99.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9L3 at UniProt or InterPro

Protein Sequence (1154 amino acids)

>BPHYT_RS33775 alpha-amylase (Burkholderia phytofirmans PsJN)
MKRDDPAETTTQSPVGNAPGNTAKPRTNRRGKPAALSDDPLWYKDAIIYQVHIKSFFDAN
NDGVGDFPGLIAKLDYIAELGVNAIWLLPFYPSPRRDDGYDIADYRNVHPDYGNLSDVKR
FIQEAHARGIRVITELVINHTSDQHPWFQRARRAKPGSNHRNYYVWSDTDQKYQETRIIF
IDSEPSNWTHDPVAGAYYWHRFYSHQPDLNFDNPAVLKEVLQVMRFWLDMGIDGLRLDAV
PYLVEREGTNNENLPETHAVLKKIRATIDAEYPNRMLLAEANQWPEDVKEYFGDEDECHM
AFHFPLMPRIYMSIASEDRFPITDIMRQTPDLAATNQWAIFLRNHDELTLEMVTDSERDY
LWNTYASDRRARLNLGIRRRLAPLMERDRRRIELINSLLLSMPGTPVIYYGDELGMGDNI
HLGDRDGVRTPMQWSSDRNGGFSRADPEQLVLPPVMGSLYGFDAVNVEAQSRDPHSLLNW
TRRMLATRRAKQTFGRGTIRFLKPENRKILAYLREMPGEPPILCVANLSRAPQAVELDLS
EFNGSVPIEMTADSVFPAIGQLTYLLTFPPYGFLWFMLCPGGQRPTWAQAHSESLPEFVT
IVIREGQVGPTPENVRLLESEVLPSWLSRRRWFASKDQKLNAVRLAALTTIPHGGFAFTE
IEADVGGHTERYVVPIAITWGGETTFPLFKQLAFARVRRGRNVGHLTDAFALPIFAHGVM
RKLRERAVVPTVQKSEIKFLPTERFAELEGLGERPEIRWLAAEQSNSSLIIADTVVLKLV
RRLVSGIHPEAEISRYLTQLGYANTAPLYGEVVRVDPEGVPHTLCILQGYIENQGDAWNW
SLDYLRRSVDELAIAVDTEAQSAPDRTNEAILLEGYSSLAGTIGRRLGELHVALASPTDD
PAFAPEPASAEQVKAWVDGTQAMLASALDLLAPRIEQMSDPETKALAQSLIDRRKELVAA
VDNLVSADAGALRIRIHGDFHLGQVLVAQGDAFLIDFEGEPARSLEERRQKSSPLRDVAG
LMRSLSYASAAAQSTTEAAPQQTADRKRALFDRFRAHATETFLSEYRAAAAEAATPLVAP
EAEQALLDLFLIEKAAYEIRYEAANRPTWLSLPVRGLAALTSRLLGDTGAPGHDSPTQAP
GAATPPNPAEGDYE