Protein Info for BPHYT_RS33770 in Burkholderia phytofirmans PsJN

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1115 transmembrane" amino acids 948 to 965 (18 residues), see Phobius details PF11896: GlgE_dom_N_S" amino acids 468 to 657 (190 residues), 180.8 bits, see alignment E=4.9e-57 PF00128: Alpha-amylase" amino acids 681 to 847 (167 residues), 33.9 bits, see alignment E=4.1e-12 PF21702: GLGE_C" amino acids 1026 to 1108 (83 residues), 56.6 bits, see alignment 3.7e-19

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_6821)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9L2 at UniProt or InterPro

Protein Sequence (1115 amino acids)

>BPHYT_RS33770 alpha-amylase (Burkholderia phytofirmans PsJN)
MEPRNGYAPRIYFFHSLLVGPLDAWPAQFAHATALGFDHALIGSLFEPGQAGHGQVVGNH
ARLHPVFEAGQQSATEAIRTLANAAKQNGLTLLADLVIDRMAADGTLYAEHADWFHPFES
EEARLDPRHVHREDNVAYANFNEAGSSAALVGWWTQQLLALAEAGVGGFRFDSPHRVPAA
VWRQLSDAVRAQHPEVRFLAATPGLARDDLLGLQGAGFDSVFSSVRWWDFRASWMVEEHA
ALARIAAPIGFPEAPYGTRLAAELTDVHDAAIVERAYRRALFTSASIGTGWMMPMGFEYG
IAEPMSQTRGDAAQFALAAQAKKFDLTEAITHVNLVASKTRTLHANGELRPLSGPGAPAA
VLLRADQPDLRDADEAILIAINPELGAPVRVDPARFLAGVPGNFTRFVPLDAPGNASPAT
LTLFSLAPGAVRLLRAVTEKPIRLAPPIDKPNSKRSGRKTVIEALSAPRVAIESVTPSVD
NGRFAAKRSVGERAEITAAIFAEGHDKIAAAVIWRAADETAWHEVPMEPAQPAGLDIWKA
RIPLDRMGRHEFTVIAWRDDFASLVEHVQKKLKAGQTVELELDEASHLFALVLAEVETTE
GAHIEPLEHIVKLFTKADAETKLALLLSPTTAKAMTAARHRPFLSRDPVIYKIDADRTAA
RFASWYEIFPRSMSDDESRHGTFADVMTKLPRIRDMGFDVLYFPPIHPIGVANRKGRNNT
LTAQPGDVGSPYAIGGVEGGHTAVHPQLGTLDDFKAMLAAAHAHGLEIALDFAVQCSPDH
PWLKEHPTWFAWRPDGTLRYAENPPKKYQDIVNPDFYAHDAKPDLWLALRDVFLFWIEAG
VHIFRIDNPHTKPLPFWEWVIADVRARYPDTIFLAEAFTRPRMMNRLGKIGFSQSYTYFT
WRESKRDFIEYMTELTQTSARDSYRPNFFVNTPDINPRHLQSQGRTGFLIRAALASTLAG
LWGVYSGFELCEAAALPNSEEYLDSEKYQLRAWDWNRPGNITGEITALNRIRRANPALQT
HLGLTFLPAHNDHILFFEKATESRDNVILVAINLDPFNEQGADIELSWETFQKWNLHDHG
PLEVTDQMTGARFEWHGRWQHVQLGSGQPFSIWRI