Protein Info for BPHYT_RS33575 in Burkholderia phytofirmans PsJN

Annotation: 1-deoxy-D-xylulose-5-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13292: DXP_synthase_N
  • amino acids 5 to 277 (273 residues), 410.4 bits, E=8.6e-127
• TIGR00204: 1-deoxy-D-xylulose-5-phosphate synthase
  • amino acids 6 to 617 (612 residues), 884.3 bits, E=2.3e-270
• PF00456: Transketolase_N
  • amino acids 25 to 182 (158 residues), 23.3 bits, E=7.7e-09
• PF00676: E1_dh
  • amino acids 110 to 182 (73 residues), 28.9 bits, E=1.5e-10
• PF02779: Transket_pyr
  • amino acids 313 to 474 (162 residues), 169.8 bits, E=1.2e-53
• PF02780: Transketolase_C
  • amino acids 502 to 611 (110 residues), 91.2 bits, E=1.2e-29

Best Hits

Swiss-Prot: 98% identical to DXS_PARXL: 1-deoxy-D-xylulose-5-phosphate synthase (dxs) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K01662, 1-deoxy-D-xylulose-5-phosphate synthase [EC: 2.2.1.7] (inferred from 100% identity to bpy:Bphyt_6781)

MetaCyc: 58% identical to 1-deoxy-D-xylulose-5-phosphate synthase (Escherichia coli K-12 substr. MG1655)
1-deoxy-D-xylulose-5-phosphate synthase. [EC: 2.2.1.7]

Predicted SEED Role

"1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)" in subsystem Isoprenoid Biosynthesis or Pyridoxin (Vitamin B6) Biosynthesis or Thiamin biosynthesis (EC 2.2.1.7)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• methylerythritol phosphate pathway II (8/9 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (10/12 steps found)
• pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (6/7 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• taxadiene biosynthesis (engineered) (10/13 steps found)
• methylerythritol phosphate pathway I (7/9 steps found)
• isoprene biosynthesis I (7/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.2.1.7

Sequence Analysis Tools

See B2T9H3 at UniProt or InterPro

Protein Sequence (635 amino acids)

>BPHYT_RS33575 1-deoxy-D-xylulose-5-phosphate synthase (Burkholderia phytofirmans PsJN)
MYDLLKTIDDPAALRRLDRRQLQPLADELRAFVLDSVSQTGGHLSSNLGTVELTIALHYV
FDTPRDRIVWDVGHQTYPHKILTGRRDQMHTLRQLGGISGFPKRDESEYDTFGTAHSSTS
ISAALGMAIASKLQGDNRTGIAVIGDGAMTAGMAFEAMNNAGVEDDVPLLVILNDNDMSI
SPPVGALNRHLARLMSGRFYAAARAGVERVLRVAPPMLDLARKLEEHAKGMIVPATLFEE
FGFNYIGPIDGHDLDSLIPTLQNIKELRGPQFLHVVTKKGQGYKLAEADPVLYHGPGKFN
PAEGIKPATTPSKKTYTQVFGEWLCDAAELDARVVGITPAMREGSGMVEFEKRFPDRYFD
VGIAEQHAVTFAGGLAAEGMKPVVAIYSTFLQRAYDQLIHDVALQNLPVVFAIDRAGLVG
ADGATHAGAYDLAFLRCIPNMTVMAASDENECRQMLYTALQQPNPTAVRYPRGAGTGVAT
VKQMAALPLGKGEIRRETSQPAGKRIAILAFGTMVAPSLAAAEQLDATVANMRFVKPLDA
ALVRQLAETHDAIVTIEEGCVMGGAGSACVEALLESGVMRPVLQLGLPDRFIDHGDPAKL
LAACGLDAAGITKSIRERFLSSGAVGGQSSVKRVA