Protein Info for BPHYT_RS33360 in Burkholderia phytofirmans PsJN

Annotation: formyl-CoA transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 TIGR03253: formyl-CoA transferase" amino acids 2 to 415 (414 residues), 730.5 bits, see alignment E=2.4e-224 PF02515: CoA_transf_3" amino acids 5 to 393 (389 residues), 323.6 bits, see alignment E=9.3e-101

Best Hits

Swiss-Prot: 70% identical to FCTA_ECOK1: Formyl-CoA:oxalate CoA-transferase (frc) from Escherichia coli O1:K1 / APEC

KEGG orthology group: K07749, formyl-CoA transferase [EC: 2.8.3.16] (inferred from 100% identity to bpy:Bphyt_6741)

MetaCyc: 70% identical to formyl-CoA transferase (Escherichia coli K-12 substr. MG1655)
Formyl-CoA transferase. [EC: 2.8.3.16]

Predicted SEED Role

"Formyl-coenzyme A transferase (EC 2.8.3.16)" (EC 2.8.3.16)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.16

Use Curated BLAST to search for 2.8.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9D3 at UniProt or InterPro

Protein Sequence (416 amino acids)

>BPHYT_RS33360 formyl-CoA transferase (Burkholderia phytofirmans PsJN)
MTKPLEGIRIIDFTHVQAGPACTQLLAWFGADVIKVERPGSGDVTRNQLRDIPDADALYF
TMLNSNKKSLTLDTKKPEGKEVLEKLIRESDVLVENFGPGALDRMGFSWERLNELNPKMI
VASVKGFSDGHHYDDLKVYENVAQCAGGAASTTGFWDGPPTISAAALGDSNTGMHLAIGI
LTALLGRDKTGKGQKVAVSMQDSVLNLCRVKLRDQQRLERVGYLEEYPQYPHGEFSDVVP
RGGNAGGGGQPGWVLKCKGWETDPNAYIYFTIQGHAWEPICKALGKPEWIDDPAYKTAEA
RQPHIFEIFKTIEGWLADKTKFEAVDILRKFDIPCAPVLTMKELANDPSLRASGTIVEVP
HKKRGSYLTVGSPIKFSDLKPEVTASPLLGEHTEEVLASLGYSKQDIFNLREVKAV