Protein Info for BPHYT_RS33350 in Burkholderia phytofirmans PsJN

Annotation: spermidine/putrescine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details amino acids 54 to 74 (21 residues), see Phobius details amino acids 86 to 105 (20 residues), see Phobius details amino acids 111 to 134 (24 residues), see Phobius details amino acids 143 to 164 (22 residues), see Phobius details amino acids 176 to 195 (20 residues), see Phobius details amino acids 226 to 248 (23 residues), see Phobius details amino acids 260 to 282 (23 residues), see Phobius details amino acids 302 to 320 (19 residues), see Phobius details amino acids 326 to 346 (21 residues), see Phobius details amino acids 358 to 381 (24 residues), see Phobius details amino acids 388 to 410 (23 residues), see Phobius details TIGR04259: oxalate/formate antiporter" amino acids 18 to 420 (403 residues), 639.4 bits, see alignment E=1.1e-196 PF07690: MFS_1" amino acids 27 to 274 (248 residues), 91.7 bits, see alignment E=2.3e-30 amino acids 266 to 424 (159 residues), 49.6 bits, see alignment E=1.4e-17

Best Hits

KEGG orthology group: K08177, MFS transporter, OFA family, oxalate/formate antiporter (inferred from 100% identity to bpy:Bphyt_6739)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9D1 at UniProt or InterPro

Protein Sequence (443 amino acids)

>BPHYT_RS33350 spermidine/putrescine ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MGHSSASERGRTAFTHHRWVQLAIGIVCMGLVANLQYAWTLFVVPMDNAHHWGQAAIQTA
FTIFIVTETWLVPVEGWLVDKFGPRPVVIGGSLCAALGWIIDAHANSLAELYAAAVIAGI
GAGCVYGTCVGTALKWFPDKRGLAAGLTAAGFGAGAAVTVIPIADMIQRSGYQHTFMFFG
IFQGVCILLLATMLIRPKPPATAVATKRIVASKIDYTPGEMIRSPLFWVLYLMFVFVAAG
GIIATAQLGPIAKEFGFAKLPVSLMGITLPLLTMTLSIDNLCNGFTRPLCGFLSDRIGRE
NTMFMIFLGEGVALLGLMQFGHNPYAFMFFAAAVFLCWGEIFSIFPATCADTFGSKYAAA
NAGTLYTAKGTASMLVPLASVLSANGGWSTVFISAAVVSIAAAVSAKFILAPMRKRWIEN
SGAPASVVIAEARLQPSSGGSPD