Protein Info for BPHYT_RS32980 in Burkholderia phytofirmans PsJN
Annotation: carboxylesterase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K03927, carboxylesterase type B [EC: 3.1.1.1] (inferred from 100% identity to bpy:Bphyt_6663)Predicted SEED Role
"Para-nitrobenzyl esterase (EC 3.1.1.-)" (EC 3.1.1.-)
MetaCyc Pathways
- methylsalicylate degradation (2/2 steps found)
- aspartame degradation (1/2 steps found)
- methyl indole-3-acetate interconversion (1/2 steps found)
- superpathway of methylsalicylate metabolism (1/5 steps found)
- retinol biosynthesis (2/7 steps found)
KEGG Metabolic Maps
- 2,4-Dichlorobenzoate degradation
- Alkaloid biosynthesis II
- Ascorbate and aldarate metabolism
- Drug metabolism - other enzymes
- Glycerophospholipid metabolism
- Retinol metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.-, 3.1.1.1
Use Curated BLAST to search for 3.1.1.- or 3.1.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2T959 at UniProt or InterPro
Protein Sequence (569 amino acids)
>BPHYT_RS32980 carboxylesterase (Burkholderia phytofirmans PsJN) MIARRHNVLEKTEPRAVLKQDASRHLPKLFVSSTLAVMLAACGGGGSAVTSDPTIAVTAD GAVKGSASDGVVAFKGIPFAAPPVGALRWRPPQPVTAWSGVRQTTSFVHDCMQTSGANSG LTVTPSEDCLYLNVWRPQNSNGKNLPVMVWIYGGAYVTGGTSMPTYDGTQFANQGVVIVT MNYRLGRFGFFAHPALTAAAAQSGETLANYGYMDQIAALKWVQRNIANFGGDPTNVTLFG ESAGGEAVHNLLTSPQATGLFAKAIIESGNGRVNQYYGRTLSRNAKTVGDSAEEQGSAFA TGYGIAGSDASSLQALRALSASQVLDGLDTSTLNSAHSLATFSGSSIIDGKLVVDEPENQ YKAGQFAKVPLLLGVNNADLGFATRGLTTKEQAYAIFGPQNLGAAAAAFDPTGTASVSTV ENQIARVITMDEPARFVARTFIAQGVPTYLYRFSYVAQSIRSKVSGATHAAEIPYVFDTL PATYGAAVTSQDEQVAHSMIAYWTAFARTGSPSGAGLTAWPRYDAGLDNQLEFTSAGTLQ INQPNPLKAQIDLVEPLNDANQTVNRQYQ