Protein Info for BPHYT_RS32670 in Burkholderia phytofirmans PsJN

Annotation: aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 100% identity to bpy:Bphyt_6600)

Predicted SEED Role

"Citrate lyase beta chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

citrate degradation (2/2 steps found)
reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

Citrate cycle (TCA cycle)

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.34, 4.1.3.6

Use Curated BLAST to search for 4.1.3.34 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TBC6 at UniProt or InterPro

Protein Sequence (270 amino acids)

>BPHYT_RS32670 aldolase (Burkholderia phytofirmans PsJN)
MSAALPRSYLFVPGNRPERFEKAHAAGADAVILDLEDAVQPAEKPAARAAVLAAVSSEAS
RPAWVRINGSDTPWFNEDVAALSGQPGVAGIVLPKAETREQIEAVLATAHAALSILPIIE
TARGFASLAVLCAAPRVQRIVFGTLDFQIDLGIDGDGEELYLFRSQIVLASRLAGIGAPV
DGVSTTITDTDAIEADARLGRRFGFGGKLCIHPKQIDAVHRAYAWSDADKAWAQRVLAAV
ETSHGAAVAVDGKMVDMPVILKARRILAGA