Protein Info for BPHYT_RS32650 in Burkholderia phytofirmans PsJN

Annotation: aldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF00171: Aldedh" amino acids 18 to 477 (460 residues), 445 bits, see alignment E=1.4e-137

Best Hits

Swiss-Prot: 51% identical to AL7A1_CAEEL: Putative aldehyde dehydrogenase family 7 member A1 homolog (alh-9) from Caenorhabditis elegans

KEGG orthology group: K00128, aldehyde dehydrogenase (NAD+) [EC: 1.2.1.3] (inferred from 100% identity to bpy:Bphyt_6596)

MetaCyc: 68% identical to L-aminoadipate-semialdehyde dehydrogenase (Pseudomonas putida)
L-aminoadipate-semialdehyde dehydrogenase. [EC: 1.2.1.31]

Predicted SEED Role

"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.22, 1.2.1.3

Use Curated BLAST to search for 1.2.1.22 or 1.2.1.3 or 1.2.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TBF7 at UniProt or InterPro

Protein Sequence (499 amino acids)

>BPHYT_RS32650 aldehyde dehydrogenase (Burkholderia phytofirmans PsJN)
MDASTILADLGIAHAAQAGDIAVHSPITGDLIGRVASNTVAEVDTALARAKEAYTAWRNV
PAPRRGELVRLLGNRLREKKQALGSIITLETGKILQEGLGEVQEMIDICDFAVGLSRQLY
GLTIASERPGHRMAETWHPMGTCVVISAFNFPAAVWSWNAALALVCGNAVIWKPSEKTPL
TALAVNQILTEALQEFGDAPAGLTALINGGRDVGAKLVADPRASIVSATGSTEMGRTVGV
EVAKRFGRSLLELGGNNAGIVTQTADHELAMRGILFSAVGTAGQRCTSLRRLFVHESVYD
KTIERLKQLYSKVPIGNPLEKGTLMGPLIDKQSYGRMQEALQQATAEGGKVFGGERVDVK
GYENGYYVRPAIVEMPSQTSVVLKETFAPILYVLRYTDFADAVEANNAAVHGLSSCVFTT
DLREAERFLSDSGSDCGIANVNIGPSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRATN
TVNYSSALPLAQGIDFNIG