Protein Info for BPHYT_RS32475 in Burkholderia phytofirmans PsJN

Annotation: dihydroxyacetone kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 TIGR02363: dihydroxyacetone kinase, DhaK subunit" amino acids 1 to 331 (331 residues), 410.4 bits, see alignment E=4.9e-127 PF02733: Dak1" amino acids 18 to 329 (312 residues), 407.1 bits, see alignment E=4.1e-126 TIGR02365: dihydroxyacetone kinase, L subunit" amino acids 366 to 562 (197 residues), 154.1 bits, see alignment E=3.9e-49 PF02734: Dak2" amino acids 389 to 562 (174 residues), 170.7 bits, see alignment E=2.9e-54

Best Hits

Swiss-Prot: 50% identical to TKFC_PIG: Triokinase/FMN cyclase (TKFC) from Sus scrofa

KEGG orthology group: K00863, dihydroxyacetone kinase [EC: 2.7.1.29] (inferred from 100% identity to bpy:Bphyt_6567)

Predicted SEED Role

"Dihydroxyacetone kinase, ATP-dependent (EC 2.7.1.29)" in subsystem Dihydroxyacetone kinases (EC 2.7.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TBC7 at UniProt or InterPro

Protein Sequence (567 amino acids)

>BPHYT_RS32475 dihydroxyacetone kinase (Burkholderia phytofirmans PsJN)
MKKLINEVSAVVPDMLDGLAALNPNLSLLQGGTIVVRADAEAAAARGEVALISGGGSGHE
PAHGGYVGSGMLSAAVAGEVFTSPSTDAVLDAIRAVAGAAGALLIVKNYTGDRFNFGLAA
EIARAEGIPTEMVIVADDVALTASGDHAGRRGLAGTVLIHKIAGAAAAAGRPLGEVAQIA
RDVAASLGTMGVALTACTVPAAGKPGFELADGEIEWGLGIHGEPGVERGALEPADAIVEK
LLAKIVGDLSLQTGERVALLVNNLGGTPSSELSIVAGSALRYLAKRGIEVERAWAGTFLS
ALEMAGVSLTLLRVDDERLAWLDAATHTSAWPALSGRVARVSVRPAPAEPERASGATLSR
EATLRRVIEAVCACLLEAEPTLTDMDQRVGDGDLGISLSRGARAILHELDDYPAETTPAA
VLRSMSATLRRVVGGTSGPLYAVMLVRAAVALEQSGGSTPKEWAVAFSAGVDGLMELGGA
HPGDRTMVDALKPAADALQSALARQDALDAALQAAVDAVSAGASQTASMHPRRGRSSYVG
DRALGHVDPGAHAVALWLAAIREALAA