Protein Info for BPHYT_RS32430 in Burkholderia phytofirmans PsJN

Annotation: formate dehydrogenase-N subunit gamma

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01583: formate dehydrogenase, gamma subunit
  • amino acids 18 to 216 (199 residues), 208.3 bits, E=6.3e-66
• PF01292: Ni_hydr_CYTB
  • amino acids 20 to 189 (170 residues), 100.8 bits, E=4e-33

Best Hits

Swiss-Prot: 52% identical to FDOI_ECO57: Formate dehydrogenase, cytochrome b556(fdo) subunit (fdoI) from Escherichia coli O157:H7

KEGG orthology group: K00127, formate dehydrogenase, gamma subunit [EC: 1.2.1.2] (inferred from 100% identity to bpy:Bphyt_6558)

MetaCyc: 52% identical to formate dehydrogenase O subunit gamma (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O gamma subunit (EC 1.2.1.2)" in subsystem Formate dehydrogenase or Formate hydrogenase (EC 1.2.1.2)

MetaCyc Pathways

• formate to dimethyl sulfoxide electron transfer (2/2 steps found)
• formate oxidation to CO₂ (1/1 steps found)
• formate to trimethylamine N-oxide electron transfer (1/2 steps found)
• nitrate reduction III (dissimilatory) (1/2 steps found)
• oxalate degradation VI (2/4 steps found)
• formate to nitrite electron transfer (1/3 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• oxalate degradation III (2/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

See B2TBB5 at UniProt or InterPro

Protein Sequence (218 amino acids)

>BPHYT_RS32430 formate dehydrogenase-N subunit gamma (Burkholderia phytofirmans PsJN)
MKHPEHTGLKDVRGNSLIVRYTPNERTNHWITAITFVLLALSGLAMFHPAMSWLYAIFGG
GQWTRILHPFVGCVMFLSFLILALRFWHHNYLDKADLQWMRQIDDVLNNREEKLPAIGKY
NAGQKLLFFTMVICLLLLLASGIVIWRRYFSFYFPIEIIRLAALVHAAAAFVLIVGIVVH
IYAALWIKGSIGAMTRGTVTYGWARKHHPNWFKEIIGK