Protein Info for BPHYT_RS32165 in Burkholderia phytofirmans PsJN

Annotation: type IV secretion system protein VirB9

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF03524: CagX" amino acids 33 to 248 (216 residues), 207.5 bits, see alignment E=1e-65

Best Hits

KEGG orthology group: K03204, type IV secretion system protein VirB9 (inferred from 100% identity to bpy:Bphyt_6499)

Predicted SEED Role

"Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9)" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T8Z2 at UniProt or InterPro

Protein Sequence (276 amino acids)

>BPHYT_RS32165 type IV secretion system protein VirB9 (Burkholderia phytofirmans PsJN)
MKTLRVSVLCLVGLLVLSPVARSWTVPGWSDDSRVRQIVYAPDAVVRINAQRGFATHIAL
DPHEQIQVVAPGDRDGWQVVANRGDHDVFLKPQLAAHNSNLEIRTDKRSYSFDLVVLPLK
AKFGNGDEIYRVTFVYPDELKAQTHAETDAALVAQRLAQPSVVRNTRYSMQVMPHSDDIA
PIAAWDDGRFTWIRIPANRRIPAIFRVADDGTESVVDKHMEDGTIVVHEVAKRFVLRLGD
EVVGIWNDAYDMDGVPPSDGTTVYGVRRVLRSLDHE