Protein Info for BPHYT_RS31615 in Burkholderia phytofirmans PsJN

Annotation: thiamine pyrophosphate protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 610 PF02776: TPP_enzyme_N" amino acids 4 to 133 (130 residues), 108.2 bits, see alignment E=4.5e-35 PF01855: POR_N" amino acids 53 to 126 (74 residues), 27 bits, see alignment E=7.2e-10 PF00205: TPP_enzyme_M" amino acids 210 to 340 (131 residues), 92.9 bits, see alignment E=2.9e-30 PF02775: TPP_enzyme_C" amino acids 401 to 556 (156 residues), 96.6 bits, see alignment E=2.5e-31

Best Hits

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 100% identity to bpy:Bphyt_6385)

Predicted SEED Role

"Pyruvate oxidase (EC 1.2.3.3)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.3.3, 1.2.5.1

Use Curated BLAST to search for 1.2.3.3 or 1.2.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T8N8 at UniProt or InterPro

Protein Sequence (610 amino acids)

>BPHYT_RS31615 thiamine pyrophosphate protein (Burkholderia phytofirmans PsJN)
MAQTVGDFIIGRLHAWGVRRIYGYPGDGINGVFGALNRAQSEARNSKSGNPTQPIEFVQV
RHEEMAAFMASAHAKFTGELGVCIATSGPGASHLITGLYDARMDHMPVLAIAGQQARAAL
GGHYQQELDLASMFKDVAGAFVEQASVPAQVRHLVDRAVRTALAERKVTALILPNDLQDL
RYEAPDRKHGTLHSGVGYRAPRLVPYPDDLQQAAEVLNAGKKVAILVGAGALHATDEVIA
IAEKLGAGVAKALLGKAALPDDLPWVTGAIGLLGTKPSYDLMMECDTLLMIGSGFPYSEF
LPKEGAARGVQIDLKADMLSLRYPMEVNLVGDSVETLRALLPLLEDKKDRAWRGKIEGWT
KDWWKTLDKRAHEAGKDAVNPQRTVWELSKRIPADSIVTSDSGSCANWYARDLKVQRGMM
CSLSDGLASMGAAVPYAIAAKFAFPERPVIALVGDGAMQMNNMAELITVSKYWKDWANPR
WICMVLNNSDLNQVTWEQRAVEGDPKFEASQDIPSVPYHKFAELIGLKGIYVDNDEQMGA
AWDEALAADRPVVIEVKSDPNIAPLPPHITLAQAKAFASTLMKGDPNEGNVIVETAKQVL
GAVLPGHHDK