Protein Info for BPHYT_RS31410 in Burkholderia phytofirmans PsJN

Annotation: D-amino acid oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 PF00890: FAD_binding_2" amino acids 19 to 259 (241 residues), 34.2 bits, see alignment E=7.7e-12 PF01266: DAO" amino acids 19 to 416 (398 residues), 245.1 bits, see alignment E=7.4e-76

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_6338)

MetaCyc: 45% identical to tyramine oxidase subunit A (Pseudomonas putida U)
Monoamine oxidase. [EC: 1.4.3.4]; 1.4.3.4 [EC: 1.4.3.4]; 1.4.3.4 [EC: 1.4.3.4]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.3.4 or 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TB64 at UniProt or InterPro

Protein Sequence (446 amino acids)

>BPHYT_RS31410 D-amino acid oxidase (Burkholderia phytofirmans PsJN)
MGPKVDTVADDIKLPERADVVVIGGGIIGVSTALALHEKGLSVAVCEKGHVAGEQSSRNW
GWVRVTRRDPREFLLSIESLKIWHTLDRTLGIDTGFNQCGILYVSNDDAVLERHRDWLKR
AREIAGDDAFDTREVDTHEVLRLLPGAKQTFKGGIFTPGDARAEPQKAAPAIANALRRKG
VSILTPCAVRGIETSGGRASAVVTEHGTIRCDAVVVAGGAWTRYFCGNLGVELPQLLTRA
SVLRTEPLEGGPTCSANNEEFAFRKRADGGYTVAYGMRTHADLTPDSFRLFFKYIEALKS
QMGALQIQIGKRFFDELKRPRRWPLNESTVFEHVRTLDPDPVVPYVDKGFKAFTEAFPQL
AGARIAQRWAGYIDVTPDAIPVISGVERVPGMFIATGFSGHGFGIGPGAGRLMADLVNHD
APLVNPHAFRLERFSDGSKITIDAGF