Protein Info for BPHYT_RS30880 in Burkholderia phytofirmans PsJN

Annotation: peptidase S13

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 544 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF02113: Peptidase_S13" amino acids 37 to 517 (481 residues), 163.5 bits, see alignment E=3.9e-52

Best Hits

KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 100% identity to bpy:Bphyt_6230)

Predicted SEED Role

"D-aminoacylase (EC 3.5.1.81)" (EC 3.5.1.81)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-, 3.5.1.81

Use Curated BLAST to search for 3.4.16.4 or 3.4.21.- or 3.5.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T8J3 at UniProt or InterPro

Protein Sequence (544 amino acids)

>BPHYT_RS30880 peptidase S13 (Burkholderia phytofirmans PsJN)
MSPTIDRQWKCWAAIASAVLLVSCGDSLSSNPTVPDSIMQVMQKPVYKNATWSMQVVDLD
SGHVIYDLNSDAQMLVGSVRKLFSVGSALNQLGAQHQFVTPVYRQGTLDAGGTLTGNLIL
LASGDLTMGGRANPDGTIAITNFDHNEANSLGNAILATPDPLAGFNSLATQVAAAGIKKI
NGDVVIDDRLFEPFDFRGEFNVRPIFVNDDVVDVSLDQSAEGSAVPFDWRPRTAAFSVQS
ALATGSAASQLDIELAPELPLCIGSPGCIGNITGNVPAGFVPPLTNAYPLIRTFRVTEPS
NYARTVFIEALARAGVSVTAAPVAANPVQLLPAKGSYSSATQVAQLTSQPYEQYVRYVMK
VSYNIGADTSLMLFGLARDGSTTLSGALAAEQAELSTTFKIASNQYHFIDGSGGGDSTAS
GTAVIAMLRGMSTTQVFSPYFDALPVLGVDGSLATITAFESDATLAGAKGQVHAKTGTYV
TENPNTPTLPFLRGQSLAGYIDAKSGHRIAFVLTVNNVPISGISDVLAVFQDEGTISAML
WKLQ