Protein Info for BPHYT_RS30520 in Burkholderia phytofirmans PsJN

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 348 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF01547: SBP_bac_1" amino acids 57 to 284 (228 residues), 49.1 bits, see alignment E=1.3e-16 PF13416: SBP_bac_8" amino acids 58 to 308 (251 residues), 145 bits, see alignment E=5.9e-46 PF13343: SBP_bac_6" amino acids 98 to 299 (202 residues), 45.4 bits, see alignment E=1.1e-15

Best Hits

KEGG orthology group: K02055, putative spermidine/putrescine transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_6156)

Predicted SEED Role

"ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T8C1 at UniProt or InterPro

Protein Sequence (348 amino acids)

>BPHYT_RS30520 ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MLRPLAAVLILGVANLAPACLTPAFAAAGKITFVSQGGTYQEAQTKAILDPAAKLLNITV
NQDSIPDAWPQIKAQAATGKPIWDVVDTPTSNCLRGGKEGLLEKLDFSKIPNAAAMPEKY
RTPYSVAYEFYSTVIGYNKKSLKKVPQSWADFWNVKAFPGTRALRNDPQTVLEAALLADG
VPRDKLYPLDVDRAFKKLQQIKPDVTVWWTSGGQSAQLLHDGEVDMTMIWNGRASAVRKD
NPDVDFTFNDGILQDTQLCVLKNAPNLPEAIKFINAAVSPELQANLPLYIDYGPGNPAAF
KTGKIDAKRASELPSSPENASKQALMSEEWWASDAGVQAKARWLKFMQ