Protein Info for BPHYT_RS30475 in Burkholderia phytofirmans PsJN

Annotation: pyruvate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF13449: Phytase-like" amino acids 58 to 427 (370 residues), 183 bits, see alignment E=6.2e-58

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_6147)

Predicted SEED Role

"3-phytase precursor (EC 3.1.3.8)" (EC 3.1.3.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T8B2 at UniProt or InterPro

Protein Sequence (494 amino acids)

>BPHYT_RS30475 pyruvate-binding protein (Burkholderia phytofirmans PsJN)
MSRSRIFALTAIASSLFFATAAHAGIDLIAVGQLSGLTGDLSKETAAPLENGVPGNLLGG
LGSGMAYAGCHTFVAVPDRGPNAVSYNPAIDDTASYINRFQTLRLRLKPGAAGASLPYTM
TPTLLATTLLHTNDKLVYGTGAAAGVPNGAPALNRRNHTNYFTGRSDNFDPTRLSTDDRD
ARFDPESVRVSNDGESVFISDEYGPYVYRFDRRSGRRIDTYKLPDAFAVATLSPIGNDEI
SQNTSGRVANKGMEGLAISPDGKTLFGAMQSPLIQDGGTKGAYTRIVRIDVRTGRTTQFA
YPLTNIGTAAKPKYPTISDVVAVNDHELLMDERDGQGLGDNSTAVFKKVFHIDLNGAQDV
SNASGEVGLAPYAVQKTLFLDVVATLTAHGYNANDIPAKLEGLAFGPDVVVGGVKKHTLF
VANDNDFLGTITDTNHPAGIANPNQFFAFAWDPSDLPGYVAQRLPGVDDDHHGDHRRDHG
DSDRMCGPERDDDH