Protein Info for BPHYT_RS30285 in Burkholderia phytofirmans PsJN

Annotation: succinate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 21 to 483 (463 residues), 560.6 bits, E=1.2e-172
• TIGR01780: succinate-semialdehyde dehydrogenase
  • amino acids 32 to 482 (451 residues), 654.2 bits, E=5.2e-201

Best Hits

Swiss-Prot: 54% identical to GABD_SINFN: Probable succinate-semialdehyde dehydrogenase [NADP(+)] (gabD) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 100% identity to bpy:Bphyt_6109)

Predicted SEED Role

"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

• 4-aminobutanoate degradation III (2/2 steps found)
• methylglyoxal degradation V (2/3 steps found)
• L-lactaldehyde degradation (aerobic) (1/2 steps found)
• L-rhamnose degradation II (5/8 steps found)
• methylglyoxal degradation IV (1/3 steps found)
• lactate biosynthesis (archaea) (2/5 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• superpathway of fucose and rhamnose degradation (4/12 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glutamate metabolism
• Pyruvate metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.22

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.22

Sequence Analysis Tools

See B2TAT8 at UniProt or InterPro

Protein Sequence (490 amino acids)

>BPHYT_RS30285 succinate-semialdehyde dehydrogenase (Burkholderia phytofirmans PsJN)
MSLALTRNELIRPQNLIDGAWTGAADGARFAVTNPATGETIVEVADSGAADARAATDAAA
RAFPAWRDTLPRERAEILRRWHALIVANTDDLAKLMSMEQGKPLAEARGEVAYGASYVAW
FADEATRIYGDLIPQQQRGKRMSAVKEPVGVIAAITPWNFPLAMIARKIAPALAAGCTVV
AKPAEDTPLTALALAVLAQEAGLPDGVLNMLSASREQGIAAVADWLADSRVRKITFTGST
PVGKHLARESAGTLKKLSLELGGNAPFIVFDDADLDAAVTGLMAAKFRNGGQTCVCPNRV
YVQAGVYERFADLLAKRVGALKVAPATDPQAQIGPMINERAIQKIARHVEDAVKHGAKVL
VGGKRLTELGPNYYAPTVLTDARDDMLVSCEETFGPVAPLFRFNEEAEAIRLSNDTPFGL
AAYFYTQDVRRINRVAAQLEAGVIGINEGAVSSEAAPFGGVKESGYGREGSKYGLDDYMS
IKYMCQGGLD