Protein Info for BPHYT_RS30155 in Burkholderia phytofirmans PsJN

Annotation: Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 TIGR02329: propionate catabolism operon regulatory protein PrpR" amino acids 10 to 660 (651 residues), 792.7 bits, see alignment E=7.8e-243 PF06506: PrpR_N" amino acids 35 to 198 (164 residues), 158.9 bits, see alignment E=3.2e-50 PF08448: PAS_4" amino acids 209 to 269 (61 residues), 26.8 bits, see alignment 1.8e-09 PF00158: Sigma54_activat" amino acids 326 to 493 (168 residues), 230.8 bits, see alignment E=2.8e-72 PF14532: Sigma54_activ_2" amino acids 327 to 498 (172 residues), 68.5 bits, see alignment E=2.6e-22 PF07728: AAA_5" amino acids 350 to 470 (121 residues), 30.4 bits, see alignment E=1.3e-10 PF02954: HTH_8" amino acids 624 to 660 (37 residues), 35.6 bits, see alignment 2.2e-12

Best Hits

KEGG orthology group: K02688, transcriptional regulator, propionate catabolism operon regulatory protein (inferred from 100% identity to bpy:Bphyt_6082)

Predicted SEED Role

"Propionate catabolism operon regulatory protein PrpR" in subsystem Methylcitrate cycle

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TAR4 at UniProt or InterPro

Protein Sequence (663 amino acids)

>BPHYT_RS30155 Fis family transcriptional regulator (Burkholderia phytofirmans PsJN)
MSTPPFEPVQRPRIWAVSISRLRDLFFDIAGEYVERADLRIVSHGFEDAVREIDAAGAGR
PDVVIAGGSNGAYLKTRVSVPVVLIGPTGFDVMHALARARRDGAKVALVTHGNTPDEVRR
FVAAYALDVTFASYQSAQHAESVVLDLRDRGIDVVVGPGMVTDLAANAGMGAVFLYSRAS
VRAAFDTALEVAQATRRETIRRQRLDNLLQHLRDGVVALDAQGRVEAMNQRLAGVLGLEA
AKAVGRVLLELAPDLAGSLPDADGDALCTVRGVSYVVHRGPLASSSAAAGTVLTFQESRA
VERLDRTLRSRQRVQQFSARYRLDDIVGASDSIERVRALVRRYARSDATVLILGESGTGK
EMVAQSMHQLSPRRDFAFVAINCGAFPEALLESELFGYEEGAFTGARKGGKAGLIEVAHR
GTLFLDEIGEMPLSLQSRLLRVLQEREVVRLGSTEPTRVDIRVVAATHRALTEGIEAGSF
RADLYYRLNILSIALPPLRERPTDLLPLAVELLLQAAAREPRLAARLPDAAAAERLLATL
AEPLKRYAWPGNVRELQNVIERIAVELADVDVDADANADVGAISAGAAAPVLTRDMLRTV
APEIVEPHARTKKAALTLRERSRHVEADEIRAALAAHDGDRDAVCEALGISKTTLWRKLS
ATQ