Protein Info for BPHYT_RS29910 in Burkholderia phytofirmans PsJN

Annotation: formyl-CoA transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 415 TIGR03253: formyl-CoA transferase" amino acids 3 to 415 (413 residues), 747.1 bits, see alignment E=2.2e-229 PF02515: CoA_transf_3" amino acids 5 to 392 (388 residues), 335.2 bits, see alignment E=2.6e-104

Best Hits

Swiss-Prot: 81% identical to FCTA_BRASB: Formyl-CoA:oxalate CoA-transferase (frc) from Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)

KEGG orthology group: K07749, formyl-CoA transferase [EC: 2.8.3.16] (inferred from 100% identity to bpy:Bphyt_6032)

MetaCyc: 64% identical to formyl-CoA transferase (Escherichia coli K-12 substr. MG1655)
Formyl-CoA transferase. [EC: 2.8.3.16]

Predicted SEED Role

"Formyl-coenzyme A transferase (EC 2.8.3.16)" (EC 2.8.3.16)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.16

Use Curated BLAST to search for 2.8.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T896 at UniProt or InterPro

Protein Sequence (415 amino acids)

>BPHYT_RS29910 formyl-CoA transferase (Burkholderia phytofirmans PsJN)
MSKALDGVRILDFTHVQSGPTCTQLLAWFGADVIKVERAGAGDITREQLRDIPDVDSLYF
TMLNHNKRSVTIDTKNPEGKQVLEALIQKCDVLVENFAPGALDRMGFTWERIQELNPRMI
VASVKGFGPGPYEDCKVYENVAQCAGGAASTTGFDDGPPVVTGAQIGDSGTGLHLALGIV
TALYQRTHSGRGQRVLAAMQDGVLNLCRVKLRDQQRLERTGVMKEYPQYPNGEFGKAVPR
AGNASGGGQPGWILKCKGWETDPNAYIYFITQAPVWARICNVIGKEEWATDPDYATPAAR
LPHLKDIFAEIERWTMTKTKFEAMQILNKYDIPCGPILSMKEIAEEPSLRKTGTIVEVDH
PTRGKYLTVGNPIKLSDSPTEVTRSPLLGEHTDEVMAELGYSPEQVSALRTAGAI