Protein Info for BPHYT_RS29905 in Burkholderia phytofirmans PsJN

Annotation: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 PF01557: FAA_hydrolase" amino acids 55 to 253 (199 residues), 200.1 bits, see alignment E=2e-63

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_6031)

Predicted SEED Role

"2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)" in subsystem Benzoate transport and degradation cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T895 at UniProt or InterPro

Protein Sequence (272 amino acids)

>BPHYT_RS29905 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (Burkholderia phytofirmans PsJN)
MDTWMRFMSSDGGVVFGRVEGGYLHEYEGLEHPVPTGAVLSTRALTPVAPCAPGKIVALW
NNFHALAAKLDKPVPAHPLFLLKPAGSVIGSGEPIRRPLSYTGKIVYEGELGIVIGRRCR
DASVEEAAAAIFGYTLVNDVTAADLLNENPHFPQWCRAKGFDTFCCVGPAIVSGFDWQQA
RLVTMLDGVERQNYPLADMVFSPAEQVSLISQDLTLEPGDVIACGTSVGVGSIKDGATVT
VTVDGIGTLSNTLSAARAGAAAGAQPVAEGAV