Protein Info for BPHYT_RS29730 in Burkholderia phytofirmans PsJN

Annotation: uracil-DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 PF13566: DUF4130" amino acids 82 to 310 (229 residues), 49.1 bits, see alignment E=6.7e-17 TIGR03914: uracil-DNA glycosylase family domain" amino acids 362 to 532 (171 residues), 208.6 bits, see alignment E=5.2e-66 PF03167: UDG" amino acids 378 to 521 (144 residues), 113.3 bits, see alignment E=1.1e-36

Best Hits

KEGG orthology group: K02334, DNA polymerase bacteriophage-type [EC: 2.7.7.7] (inferred from 100% identity to bpy:Bphyt_5994)

Predicted SEED Role

"Domain often clustered or fused with uracil-DNA glycosylase / Uracil-DNA glycosylase, putative family 6"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T859 at UniProt or InterPro

Protein Sequence (546 amino acids)

>BPHYT_RS29730 uracil-DNA glycosylase (Burkholderia phytofirmans PsJN)
MKRIAIEPSFAAWRHAARELLRQGVEPERIEWVECDPCDSAGSDSGNASAQQGSATAQAT
PTPAIPRELLAWLKTAACYYAPDRWPLLYRILWRWTHGERHVLDLQDVDGALLDQRIQSV
EHEISDLVALTLFRRRDPSMGAPEFVGWYEPHHDLLAQAAERFAERMGDSTWMLATPQGA
AFWNGMLLQISRPATEENGHVTHVLPAGQLNGPATPALPPGQRKGPTTPTLPKSQPNGPT
APTLPPSQRNGPTTPTLPPSQRSEPTTPTLPRAAMAGEATTSEPTEALWLAYYASVFNGA
PTPMPLRYWKTPPAGPPLPARLARERSRLGAQSGTVTIPETPPLEYSAVTPPLREPTGPL
PTCRRCALWRNAKQAVAGAGPARAALMVVGEQPGEHENQHGVPFTGPAGQLLDTVLVRAG
LERSALYLTYAVKHYKWETLEQRRVHRTPARREVEACQYWLDHELAQVAPRVVVTLGATA
LKALTGAHVNLSEYLGQTIDHGGRLIVPTWHPSYALKMADGRLREDIVAGMVAAFRHAAG
LAAEGA