Protein Info for BPHYT_RS29590 in Burkholderia phytofirmans PsJN

Annotation: glycogen phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 817 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 16 to 811 (796 residues), 1173.9 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 104 to 811 (708 residues), 1071.2 bits, see alignment E=0

Best Hits

Swiss-Prot: 55% identical to PHSG_HAEIN: Glycogen phosphorylase (glgP) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to bpy:Bphyt_5966)

MetaCyc: 51% identical to glycogen phosphorylase b (Homo sapiens)
Phosphorylase. [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T832 at UniProt or InterPro

Protein Sequence (817 amino acids)

>BPHYT_RS29590 glycogen phosphorylase (Burkholderia phytofirmans PsJN)
MTAVDLEFDQLNSTVDALRRSISNRMMYGVGKDAVTAHPHDWLHAAALAVRDRLVARWMK
TTRLQYEQDVKRVYYLSMEFLIGRTFTNALLALGIHDQMKEALASLGVDMDALTDIEPDA
ALGNGGLGRLAACFLDSMATLGIPGFGYGIRYEYGMFRQQIVNGEQVEAPDYWLRAGNPW
EFPRPEVTYMVHFGGRTVQRGEHVEWIDTQHVNATAYDTVIPGYATDATNTLRLWSARAA
EELDLGAFNRGDYRNAVDTKNMSENVSRLLYPDDSTPAGRELRLRQEYFFVSATMQDLIR
RYQRTHSTFGRFSEKVAVHLNDTHPVLAIPELMRLLVDVHHLQWDKAWKHVTQIFSYTNH
TLMPEALETWDVELLARLLPRHLEIIFEINAQFLKHVSEQSGHDAEMIRRISLVDEYGQR
RVRMAYLAIVASHKVNGVSKLHSQLMTRDIFADFARVFPDRFTNVTNGITPRRWLAQASP
SLSSLIDQQIGKHWRSNLFELEQLRNLRTDSGFIEAFREAKRQNKLRLVHRLAHHTKLHF
DPDALFDLQVKRIHEYKRQLLNVLHVIVRYNQIRANPERDWVPRVVMFAGKAASAYRMAK
TIIKLIGDVSEKVNHDPLVGDRLKVVFVPNYGVSVAELIIPAADLSEQISMAGTEASGTG
NMKLALNGALTIGTMDGANIEICDAVGRENIFIFGHTADEVDNLRATGYRPRQIYEENAE
LRTALDQIRTGFFSPDDPLRFSDIFHTLVDWGDHYMVLADFAAFAKAQDEVDARFVDKRA
WTESAIENVAGMGQFSSDRTIGEYARNIWHVNPLNIE