Protein Info for BPHYT_RS29570 in Burkholderia phytofirmans PsJN

Annotation: ABC transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF00497: SBP_bac_3" amino acids 31 to 260 (230 residues), 159.7 bits, see alignment E=7.9e-51 PF09084: NMT1" amino acids 77 to 176 (100 residues), 28 bits, see alignment E=1.9e-10

Best Hits

Swiss-Prot: 56% identical to GLTI_ECOLI: Glutamate/aspartate import solute-binding protein (gltI) from Escherichia coli (strain K12)

KEGG orthology group: K10001, glutamate/aspartate transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_5962)

MetaCyc: 56% identical to glutamate/aspartate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-13-RXN [EC: 7.4.2.1]; 7.4.2.1 [EC: 7.4.2.1]

Predicted SEED Role

"Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4)" (TC 3.A.1.3.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T828 at UniProt or InterPro

Protein Sequence (291 amino acids)

>BPHYT_RS29570 ABC transporter (Burkholderia phytofirmans PsJN)
MALATFAVVSTSASAQETQSTLAKIRSGNSISIGHRETSVPFSYVDTNNQVIGFSQDICN
RIIDAVKAKTGHPDLHVRFIPVTSQNRIPLVQNGTVDLECGVTTNLTSRQNQVAFSDTFF
VATTRLLTRKDSGIKDFTDLAGKTVVTNQGTTSERLLRKMNEEKKMNMQIISAKDYGEGR
VTLETGRAVAYMMDDVLLAGARSLTAKPADWVIVGAPQSSEAYGFMLRKDDPEFKRLVDD
AMVRVMKGSEIKTLYDKWFLKPVPPKGINFDFPMSEALEKLYATPNDRAFD