Protein Info for BPHYT_RS29555 in Burkholderia phytofirmans PsJN

Annotation: MexH family multidrug efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 50 to 364 (315 residues), 226.3 bits, see alignment E=2.3e-71 PF16576: HlyD_D23" amino acids 74 to 275 (202 residues), 95.4 bits, see alignment E=4.7e-31 PF13437: HlyD_3" amino acids 171 to 272 (102 residues), 72.7 bits, see alignment E=5.5e-24

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5959)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T825 at UniProt or InterPro

Protein Sequence (383 amino acids)

>BPHYT_RS29555 MexH family multidrug efflux RND transporter periplasmic adaptor subunit (Burkholderia phytofirmans PsJN)
MTERKPMTKRMVIMLICVGLLLAALVGFNVFRAHMIAKFMSGNAAPPATVSAVVARYQQW
QPQLAAVGSLRAVRGVDVTTEVAGLVREVAFSSGQEARAGQVLIRLNADSDVALLRSLEA
SAELAQTVYTRDKAQYDIKAIAKAQLDADAADLKVKKAQVAQQAALVDKKTIRAPFAGRL
GITTINPGQYINPGDAIVTLQAIDPIYADFYLPQQQLGQLAIGQTIVVDTNAYSGQTFVG
KIQSVSPKVDSTTRNVQIEASVDNRERKLLPGMYANVKIDAGAGQRYLTLPQTAITYNPY
GATVFIVKPGTQPNAQGKALPVAQQVFVTPGPTRGDQVAILKGVDDGAQVVTSGQLKLKN
GTPLVINNSVQPADDPNPTPQEQ