Protein Info for BPHYT_RS29525 in Burkholderia phytofirmans PsJN

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01096: lysine-arginine-ornithine-binding periplasmic protein" amino acids 3 to 253 (251 residues), 279.7 bits, see alignment E=1.1e-87 PF00497: SBP_bac_3" amino acids 28 to 254 (227 residues), 213.1 bits, see alignment E=1.8e-67

Best Hits

Swiss-Prot: 54% identical to ARGT_SALTY: Lysine/arginine/ornithine-binding periplasmic protein (argT) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K10013, lysine/arginine/ornithine transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_5953)

Predicted SEED Role

"Histidine ABC transporter, histidine-binding periplasmic protein precursor HisJ (TC 3.A.1.3.1)" in subsystem Arginine and Ornithine Degradation (TC 3.A.1.3.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T819 at UniProt or InterPro

Protein Sequence (260 amino acids)

>BPHYT_RS29525 ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MKKLLAALTVALLATVSIGAHAKDWSTVRFGVDASYPPFESKGSDGKLVGFDIDLGNEIC
ARMKAKCVWVENDFDGMIPALKAKKFDGVLSSMSMTPQRAEQIAFSNKLFNTPTRLVAKK
GSGILPTADSLKGKTVGVEQGTIQETYAKTYWEPKGTKVVPYQNQDQVYADLLSGRLDAA
LQDAVQAEIGFLKTPRGAGFDFVGKNLDDPKILGNGAGIGMRKEDTDLKAKVDKAIADII
KDGTYKKLEKKYFDFDVYGG