Protein Info for BPHYT_RS29360 in Burkholderia phytofirmans PsJN
Annotation: acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to ACOA_CUPNH: Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha (acoA) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)
KEGG orthology group: K00161, pyruvate dehydrogenase E1 component subunit alpha [EC: 1.2.4.1] (inferred from 100% identity to bpy:Bphyt_5920)MetaCyc: 66% identical to acetoin:DCPIP oxidoreductase alpha subunit (Syntrophotalea carbinolica DSM 2380)
RXN-9718 [EC: 2.3.1.190]
Predicted SEED Role
"Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-)" in subsystem Acetoin, butanediol metabolism (EC 1.2.4.-)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Lysine degradation
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.-, 1.2.4.1
Use Curated BLAST to search for 1.2.4.- or 1.2.4.1 or 2.3.1.190
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2TG02 at UniProt or InterPro
Protein Sequence (327 amino acids)
>BPHYT_RS29360 acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha (Burkholderia phytofirmans PsJN) MSVSTELSKEDLLKAYRMMRTIREFEERLHVEFATGEIPGFVHLYAGEEASAVGTMLHLN DADYVATTHRGHGHCIAKGVDVRGMMAEIYGRKTGVCHGKGGSMHIADLSKGMLGANGIV GAGGPLICGAALAAKYKKSGGVGVCFFGDGASNQGVIFESLNLASVWRLPAIFVAENNGY AEATSSTWSVATDNIADRASGFGMPGVIVDGFDFFAVHEALGEAIERARQGGGPTLVEVK FSRYFGHFEGDAQTYRAPGEVQKLRDEKDCLKRFEERVVRAEMLSVDQLRGVDAEVKSLI DSAVTEAKAAPLPTAEDLLSDVYVSYP