Protein Info for BPHYT_RS28945 in Burkholderia phytofirmans PsJN

Annotation: endo-1,4-D-glucanase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01270: Glyco_hydro_8" amino acids 34 to 369 (336 residues), 212.6 bits, see alignment E=3.5e-67

Best Hits

Swiss-Prot: 44% identical to GUN_PSEFS: Endoglucanase (bcsZ) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01179, endoglucanase [EC: 3.2.1.4] (inferred from 100% identity to bpy:Bphyt_5838)

Predicted SEED Role

"Endoglucanase precursor (EC 3.2.1.4)" (EC 3.2.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TFR8 at UniProt or InterPro

Protein Sequence (393 amino acids)

>BPHYT_RS28945 endo-1,4-D-glucanase (Burkholderia phytofirmans PsJN)
MRVRINKKIGVALALGLAASVSFANLVKVAQTTAAPGACGDWSGYRAFVERFVQADGRVI
DYSTPAQQTTSEGQSYALFFALVANDRATFDRLLGWTRTNLAGNQFDAQNMRLPSWQWGR
KADGSYGVLDPNSASDSDLWIAYDLLQAGRLWHEAAYTQLGEALAAQIARQEMTTLPGVG
PMLLPGPQGFKSGGVTRLNPSYLPLPVLRSLAHDMPNGPWGKLADSAYKLIKTTAPQGFA
PDWAAWQNGQFVVDPKNGDTGSYDAIRVYLWAGLASPADPLAKPWLAALGGMRARVAQTG
FPPEKVSSTSGTASGEGPLSYWAALAPYFKALGDERGLGLARTHLAALDTNVPGREPVYY
DRVLGLFGTGFIDGRYRFDEAGRLVPSWRKECD