Protein Info for BPHYT_RS28690 in Burkholderia phytofirmans PsJN

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 signal peptide" amino acids 1 to 42 (42 residues), see Phobius details transmembrane" amino acids 223 to 244 (22 residues), see Phobius details amino acids 339 to 355 (17 residues), see Phobius details PF00563: EAL" amino acids 257 to 483 (227 residues), 127.2 bits, see alignment E=3.6e-41

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5786)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TGF2 at UniProt or InterPro

Protein Sequence (518 amino acids)

>BPHYT_RS28690 diguanylate phosphodiesterase (Burkholderia phytofirmans PsJN)
MAFAFPVSTISRSAFSALRRASERGLAVVLVICTCTSLALAGPADTSSGPILTIERNVWQ
QLTDWLAGPPAPPIRFGSACAPIAQLPVAPAALAGPDAGPMPVLTDSCDLLKDERDVASG
TEAASVSPKPNVVTLLREVPWQIGDQAAAERNGFPASDTVAGLTSRADGAEQSPEQPAMS
VLDCSVGRSCETQPISPRIGDDKLAPARAPATTATQRTSAWDLLGFVLWPMLGAILVVVV
GWALKRWFRYDKSLLRAARAGLRRSEFHLEYQPVVGVRRARCVGVEALLRWDNQKYGALG
PAHYMEFIENSSLIGPMTRFVMSRAAQELREIGAPKSLYLGVTAPASYLVSSAFIAHLNE
VGSVGLPPLILKIEAGSARKFKKRLVPMMAQARDRGVRFALSAVRPTDAAIELPEEMTFE
MVKIDRNVLGMDPDERSRQIGALTHMGHEMGAVVVVEGIENSAHHNVARASRAEFGQGFF
YSRALGASRLKVFLEAANAPSSKPGGTANLLGWRVRNF