Protein Info for BPHYT_RS28580 in Burkholderia phytofirmans PsJN

Annotation: 3-beta hydroxysteroid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 PF05368: NmrA" amino acids 3 to 71 (69 residues), 29.7 bits, see alignment E=6.7e-11 PF01370: Epimerase" amino acids 3 to 240 (238 residues), 52 bits, see alignment E=1e-17 PF13460: NAD_binding_10" amino acids 7 to 74 (68 residues), 39.6 bits, see alignment E=7.9e-14

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5761)

Predicted SEED Role

"Nucleoside-diphosphate-sugar epimerases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TGD1 at UniProt or InterPro

Protein Sequence (323 amino acids)

>BPHYT_RS28580 3-beta hydroxysteroid dehydrogenase (Burkholderia phytofirmans PsJN)
MHVFVTGGTGHAGSHIIPDLVAAGHEVTALARTDESAATVSALGAKARRGDLSDLAGLKA
AAAESDGVIHVGYRADLLQAGGIAALGDSELSIVLAFGEALAGTGKPLVVAGSIGAPTNV
GRGAPLVAPVSLGRPATEDDPALPGRPLDAGTLRARNVVETAVVGLAEQGVRSSVVRIPL
IAHSTADRVGFLQILIGLAKEKGVIGYPGDGENRWPAVHIRDLASLFRLALEKGPAGKTW
HAVADEGIRFREIAEAIAARLGVPAVSIPADELMVPGYFGFLSAVVTGDFPASNAITRKS
LGWEPAQPGLLEDMDNGHYFPAG