Protein Info for BPHYT_RS28520 in Burkholderia phytofirmans PsJN

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 534 PF08448: PAS_4" amino acids 2 to 102 (101 residues), 49.1 bits, see alignment E=1.2e-16 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 108 to 267 (160 residues), 131.1 bits, see alignment E=1.7e-42 PF00990: GGDEF" amino acids 112 to 265 (154 residues), 135.5 bits, see alignment E=2.9e-43 PF00563: EAL" amino acids 286 to 524 (239 residues), 233.5 bits, see alignment E=4.7e-73

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5749)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TGB9 at UniProt or InterPro

Protein Sequence (534 amino acids)

>BPHYT_RS28520 hypothetical protein (Burkholderia phytofirmans PsJN)
MLDHITDGVMSLDREWKFRNINRTAARLLKRRPADLLGQEIWAEYPDLIGSSYETAYRQA
AETGLPSSATAFYAPLDTWFEVRAFPCEDGLIVLVRDVTEARAISELLSRQATHDELTGL
INRRELLLRLNRLIDEGAADSVDLLFIDLDRFKDINDSFGHAAGDEVLRVVGERLSDLLT
ERIHASRIGGDEFVIFLVDAPEGEAVRVAREVIVRMREPVQTPSVRAPVGASIGVARYPV
SASDAGELLRNADTAMYHAKRAGGSQVWSFGKEDAQRLAHRLRLRADLESASASHQFELH
YQPQLDLGTGRVYGAEALLRWNHPKLGLLTPGDFLDVLLESPAYEATGEWLIRRAFRQAA
QWQAPDHAPFKIAVNLSATTIQNCDLAALVSEAAEASGVSASVLEIEVTETVVMSDFVAA
SRSLSAVRRLGVTVSLDDFGTGYSSLAYLTQLPVDRIKIDKSFVQRLTVEETCHQARAMV
EAMVTLAHALGMGTVAEGVETETQLALVRELGCDAAQGYFIGQPMPAARMEPYL