Protein Info for BPHYT_RS28340 in Burkholderia phytofirmans PsJN

Annotation: indole acetimide hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 PF01425: Amidase" amino acids 31 to 466 (436 residues), 306.5 bits, see alignment E=2e-95

Best Hits

KEGG orthology group: K01463, [EC: 3.5.1.-] (inferred from 100% identity to bpy:Bphyt_5709)

Predicted SEED Role

"Indoleacetamide hydrolase (EC 3.5.1.-)" in subsystem Aromatic amino acid degradation (EC 3.5.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.-

Use Curated BLAST to search for 3.5.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TFP7 at UniProt or InterPro

Protein Sequence (484 amino acids)

>BPHYT_RS28340 indole acetimide hydrolase (Burkholderia phytofirmans PsJN)
MTWTVDEQLALSATEAVAAIQSGRLNAADYVATLLARAAALSSLNALTTLELDGALAAAR
RIDALPAAEKARLPLAGLPIVVKDNINTAGMQTSAGTPALAGFIPKTNAPSVQRLIDAGA
IVLGKANMHELAFGITSTNLATHAGPVRNPYDPSLIPGGSSGGTAAAIAARIVPAGLGTD
TGGSTRIPAALTGTVGFRPSVGNGGAERRYHDPDAVVPISHTRDTVGPMARTVADIALLD
GVITGAGALPVVALNGLRIGLPAPLWEGLERQVEDVARAALRQLEAAGVVFVPVAMSELD
DLNGMVGGPIAIHEARDDVLAWLVANDAPVKTVVEMAARIASPDVRAIYDGVLSDVLGDR
YEAALNHWRPRLQQYMAATFADERLDALLFPTTRLAAVPIDEVNGSSVVSVDGSAAIDTM
DAFLHNTDPASTSGIPGLSLPAGMTASGLPVGLELDGPLGEDRRLLAIGIAFEQLLGALP
APVL