Protein Info for BPHYT_RS28265 in Burkholderia phytofirmans PsJN

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 86% identical to T3HPD_BURCH: Trans-3-hydroxy-L-proline dehydratase (lhpH) from Burkholderia cenocepacia (strain HI2424)

KEGG orthology group: K01777, proline racemase [EC: 5.1.1.4] (inferred from 100% identity to bpy:Bphyt_5694)

MetaCyc: 85% identical to trans-3-hydroxy-L-proline dehydratase subunit (Azospirillum brasilense)
Trans-L-3-hydroxyproline dehydratase. [EC: 4.2.1.77]

Predicted SEED Role

"Not a Proline racemase, nor 4-hydroxyproline epimerase [missing catalytic residues]" in subsystem Proline, 4-hydroxyproline uptake and utilization

MetaCyc Pathways

trans-3-hydroxy-L-proline degradation (2/2 steps found)
L-proline degradation II (reductive Stickland reaction) (2/3 steps found)

KEGG Metabolic Maps

Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.77 or 5.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TFN2 at UniProt or InterPro

Protein Sequence (335 amino acids)

>BPHYT_RS28265 hypothetical protein (Burkholderia phytofirmans PsJN)
MKISRTIQTVEVHTGGEAFRIVTSGLPRLPGDTIVKRRAWLKENADDIRRALMFEPRGHA
DMYGGYLTEPVSPNADFGIIFLHNEGYSDHCGHGVIALSTAAVELGWVQREIPETRIGID
APCGFIEAFVEWDGEHAGNVRFVNVPSFIWKRDVTVETPSFGSVTGDIAFGGAFYFYTDG
EPHGLAVRESSVEELIRFGAEVKQAANQAFPVEHPYIPEINHIYGTIIANAPRHAGSTQA
NCCVFADREVDRSPTGSGTGGRVAQLYLRGKLGKDDTLVNESIIGTVFKGRVLSETTVGD
FKAVVPEVEGNAFVCGFATWIIDERDPLAYGFLVR