Protein Info for BPHYT_RS28085 in Burkholderia phytofirmans PsJN

Annotation: Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 PF01590: GAF" amino acids 62 to 190 (129 residues), 50.7 bits, see alignment E=6.7e-17 PF00158: Sigma54_activat" amino acids 336 to 497 (162 residues), 217.5 bits, see alignment E=2.5e-68 PF14532: Sigma54_activ_2" amino acids 337 to 503 (167 residues), 74.5 bits, see alignment E=2.5e-24 PF07728: AAA_5" amino acids 355 to 466 (112 residues), 23.9 bits, see alignment E=9.3e-09 PF02954: HTH_8" amino acids 624 to 658 (35 residues), 32.4 bits, see alignment (E = 1.6e-11)

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5659)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TFJ8 at UniProt or InterPro

Protein Sequence (663 amino acids)

>BPHYT_RS28085 Fis family transcriptional regulator (Burkholderia phytofirmans PsJN)
MTQRSVTPPAVGRPDVIAQAHARSLEIGLRASEAPDFHPLPRPALRELVERNQSLFTHAL
PVMETLHAQIVDTQSMVLLTDNHGVILHSLGDSDFVEKANRVALCPGVSWAEADRGTNAI
GTALVDGQPTVVHAGEHFLHANRILTCSCAPIADPFGRTIGALDVSGDTRGFHKHTLALV
RMSAQMIENHLFSNQFADAIRVHFHARGEFIGTLFEGLAAFAPDGTFLSANRSALFQFGQ
ALAELQRQPFDALFGVPISRLLQQIARAPGENIQLTLPSGVRVVARGEYALPRYVAPSES
LAGTSRAPLSSDARHAPRVADPAPPATLETLDTGDAQVAAILRRVAKLRGRDIPILVLGK
TGTGKEWLARAIHHDSPRRDAPFIALNCASLPETLIEAELFGYEDGAFTGAKKRGSVGKI
VQADGGTLFLDEIGDMPLAQQVRLMRVLQERNVVPLGGTRAIPVDLRIVCATHRNLRAMI
EAGTFREDLYYRINGLVLTLPALRERTDLRALVTRMLELQPAGERLPRRVSADVLERFAQ
CRWPGNLRQLANVLRTASIMAEGAEQIELDDLPEDFLQDCIDTAAEPCVQSPAMRADSGS
CDEVRAAAQTQGQPATSKMEAWQATLIAQTLERLDGNVSAAARELGLARNTVYRYLRRGG
STH