Protein Info for BPHYT_RS27880 in Burkholderia phytofirmans PsJN

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1059 transmembrane" amino acids 20 to 39 (20 residues), see Phobius details amino acids 304 to 325 (22 residues), see Phobius details PF00672: HAMP" amino acids 324 to 374 (51 residues), 40.1 bits, see alignment 1.3e-13 TIGR00229: PAS domain S-box protein" amino acids 502 to 625 (124 residues), 51.7 bits, see alignment E=9.2e-18 PF00989: PAS" amino acids 510 to 616 (107 residues), 23.8 bits, see alignment E=1.3e-08 PF08448: PAS_4" amino acids 511 to 620 (110 residues), 27.5 bits, see alignment E=1.1e-09 PF13426: PAS_9" amino acids 516 to 618 (103 residues), 41.9 bits, see alignment E=3.7e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 627 to 791 (165 residues), 139.6 bits, see alignment E=8e-45 PF00990: GGDEF" amino acids 630 to 787 (158 residues), 157.9 bits, see alignment E=6.8e-50 PF00563: EAL" amino acids 808 to 1042 (235 residues), 252.2 bits, see alignment E=1.6e-78

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5615)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TG91 at UniProt or InterPro

Protein Sequence (1059 amino acids)

>BPHYT_RS27880 diguanylate phosphodiesterase (Burkholderia phytofirmans PsJN)
MKRNLLVHLLGRLRVGRKLLLIYLLDLSAVVFISGILIHEKYIAIDFSDKELVGNAYIAA
AQDALTGFALAGAGRQQSAAQLQQTAGKLADAERRFGEHMQSAALNQRVRDALLASASPH
ADAATLDGGLEATRALVTRVGNQSNLILDPDLDSYYAMSLSILRYPELLSDVDRIGRHLH
DAGAKSRTVPMGRDEMRTRYLVLEGQLDAVLQGLRSDYAEASAANGTLTRTIGPSIERLL
AAIDSYRRVAGAAVDAGGDVPELATLDAAQQRVVISVSDAWSITSSQLERLLHERVRGLF
ERMWLHLGTALFLLCAILSMVYAVAQQISRPLRELARVMDTVRRTGDHSLRADWHSQDEI
GRLVRGFNDMLAQLGRERDIQKELAATARASAAQYALVEATPVPMVVTAIPGHEVLHANR
PALAWLNGCTVDPWGYGLDSEVRARFFQQLSDRDAVDEFEVRWKASNEPSWAMLSARRLA
FQGRDAVLTAFTPINQIKSMEQRLELWGKVFEASSEAILILDAQRRLVTANASFYRATGY
RADDVAGKEPAFLSGAHVQGDLLAAVIDSVDRQSAWHGEANVRRRAGSDYPAWLMISAVR
DRRASLSHYICTLIDITDRKKSEARIQFLAEHDLLTELPNRALFMKRLRAVLDHARVADH
RVAVLFIDLDRFKHVNDSLGHHIGDSLLRSVSRRLVRAVRADDTVSRLSGDEFTIILNGV
SGAAEVGRTIEDRLLPLMRQPHELDGMALPVSCSVGVALFPDDGEDIETLMQNADAAMYQ
AKGAGRNLVKFFTPDMAERALHRAQLEASLRNAIAMDEFRLEYQPCIDARTGELVAVEGL
LRWHNPRLGKVSPAEFIPIAEESRLIISMGAWVIEQACRQIAEWNDEGLAPLRVSINVSA
IQLRDPNLIGMLATSLERNRVAPERLELEITETVLMDSAEHYESTVAGIRKLGIKLSLDD
FGVGYSSLSYLNRFPLDRLKIDRAFVKDMLDAPADLAIIRAIIELGHELGLRVVAEGVES
EHQAQILRNIGCDELQGFLFSPSLSPADLGSWSSMRMVA