Protein Info for BPHYT_RS27750 in Burkholderia phytofirmans PsJN

Annotation: RND transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details transmembrane" amino acids 155 to 172 (18 residues), see Phobius details amino acids 193 to 213 (21 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 29 to 493 (465 residues), 377.9 bits, see alignment E=3.6e-117 PF02321: OEP" amino acids 93 to 282 (190 residues), 68.1 bits, see alignment E=4.6e-23 amino acids 306 to 491 (186 residues), 77.2 bits, see alignment E=7.5e-26

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5589)

Predicted SEED Role

"RND efflux system, outer membrane lipoprotein, NodT family" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TG65 at UniProt or InterPro

Protein Sequence (498 amino acids)

>BPHYT_RS27750 RND transporter (Burkholderia phytofirmans PsJN)
MSTLPPDSAPRARRADTVRRRVARAWALLLALLLNACSVGPDFSRPPSPGVDRFVQGDAL
RTPLQAAGSVQTFSAQTNVPSDWWTRFGSLAIDSAVDEALTGNATIEGAQAALRQSEDEM
RAGAGVFYPQVDAGFAGSRQKYSPSRIGANEPSTLFNLFTLSASVSYALDIWGGQRRMVE
ALQARVDAQRYTVLATYLTLTVNVVNAMIARAAYRDEIAATREMTALVHEQIRITQAQVE
AGASAYAAVLTLENELATLEASLPALEQKRAQAEDLLASLSGMLPVEWHAPDLSLEDITL
PHTLPETVPASLVRRRPDILQAEAALHVASANIGVATANLLPGVTLSASGGYDNTSMSAL
LHRSGQIWTIAADVSAPVFHGGTLWFQRRAALNAYAQTRASYRQVVLSAFAQVADTLRAL
ENDAQALDAQTRARDAAAEALRLVEADYAAGTVGYVQTLIADGLFHQARIAWLQGVAQRL
QDTVALYAALGGGWESNQ