Protein Info for BPHYT_RS27640 in Burkholderia phytofirmans PsJN

Annotation: acetoacetyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 248 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01829: acetoacetyl-CoA reductase" amino acids 5 to 247 (243 residues), 326.4 bits, see alignment E=6e-102 PF00106: adh_short" amino acids 6 to 197 (192 residues), 191.1 bits, see alignment E=2.3e-60 PF08659: KR" amino acids 8 to 166 (159 residues), 74.7 bits, see alignment E=1.3e-24 PF13561: adh_short_C2" amino acids 15 to 246 (232 residues), 196.7 bits, see alignment E=7.1e-62

Best Hits

Swiss-Prot: 51% identical to PHAB_CUPNH: Acetoacetyl-CoA reductase (phaB) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00023, acetoacetyl-CoA reductase [EC: 1.1.1.36] (inferred from 100% identity to bpy:Bphyt_5566)

MetaCyc: 51% identical to hydroxyvaleryl-CoA reductase / acetoacetyl-CoA reductase (Cupriavidus necator)
Acetoacetyl-CoA reductase. [EC: 1.1.1.36]; 1.1.1.36 [EC: 1.1.1.36]

Predicted SEED Role

"Acetoacetyl-CoA reductase (EC 1.1.1.36)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle (EC 1.1.1.36)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.36

Use Curated BLAST to search for 1.1.1.36

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TG44 at UniProt or InterPro

Protein Sequence (248 amino acids)

>BPHYT_RS27640 acetoacetyl-CoA reductase (Burkholderia phytofirmans PsJN)
MSAKRVAFITGGMGGLGAAVSRRLHDAGMVVAMSHSERNDHVATWLMHERDAGRRFHAYE
ADVTSFDSCAHCAARVLDEFGSVDVLVNNAGIARDSTFAKMSKDDWDAVLRTDLDSMFNM
TKPLLGSMVAQRFGRIVNIASVNGARGAFGQTNYAAAKAGIHGFTMSLALEVAKHGVTVN
TVSPGYLATAMTAAVPKEVMETKILPQIPVGRLGQPDEVAALVAFLCSDDAAFVTGANFA
INGGMHMG