Protein Info for BPHYT_RS27580 in Burkholderia phytofirmans PsJN

Annotation: F0F1 ATP synthase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 transmembrane" amino acids 438 to 456 (19 residues), see Phobius details TIGR00962: ATP synthase F1, alpha subunit" amino acids 21 to 471 (451 residues), 556.8 bits, see alignment E=2.4e-171 PF02874: ATP-synt_ab_N" amino acids 31 to 95 (65 residues), 25.9 bits, see alignment E=1.7e-09 PF00006: ATP-synt_ab" amino acids 152 to 367 (216 residues), 255.4 bits, see alignment E=6.4e-80 PF00306: ATP-synt_ab_C" amino acids 374 to 490 (117 residues), 73.4 bits, see alignment E=3.3e-24

Best Hits

Swiss-Prot: 84% identical to ATPA1_PARXL: ATP synthase subunit alpha 1 (atpA1) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K02111, F-type H+-transporting ATPase subunit alpha [EC: 3.6.3.14] (inferred from 100% identity to bpy:Bphyt_5553)

MetaCyc: 49% identical to ATP synthase F1, alpha subunit (Synechococcus elongatus PCC 7942 = FACHB-805)

Predicted SEED Role

"ATP synthase alpha chain (EC 3.6.3.14)" in subsystem F0F1-type ATP synthase (EC 3.6.3.14)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TG33 at UniProt or InterPro

Protein Sequence (522 amino acids)

>BPHYT_RS27580 F0F1 ATP synthase subunit alpha (Burkholderia phytofirmans PsJN)
MFPPETADDPWLAQQRLTLARTSITAQAETLGRVERVGDGIAFVSGLPDAALNELLRFES
GATGFAHTLDADEVSVVLLDNDGAIEAGARVTRTGTVLEVPVGEGLLGRVIDPLGRPLDR
DEPIVAAMRMPAERPAPSIVERDLVSEPVETGVLLVDALFAIGRGQRELIIGDRATGKTS
LAIDAIVNQKQSDMICVYVAIGQRTTAVQRVIEAVRQYGAPERCIFVVATAASAPGLQWI
APFAGITVAEYFRDRGQHALVVIDDLTKHAATHRELALLTREPPGREAYPGDIFYVHARL
LERAARLSTKLGGGSLTALPIAETDAGNLSAYIPTNLISITDGQIVMDAALFAANQRPAV
DIGLSVSRVGGKAQHPALREVSGRLRLDYAQFLELEMFSRFGGLTDAHVAGKIARGQRIR
ALIAQPRFSALRTVDEIALLAALAAAVFDAVPAGIAAQVRTELVARAQGQHVRSELPQAC
ARISAKSSDTDTPAELIATVRELVQQLTLRAANVASDLDLAR