Protein Info for BPHYT_RS27240 in Burkholderia phytofirmans PsJN

Annotation: N-ethylmaleimide reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 PF00724: Oxidored_FMN" amino acids 3 to 346 (344 residues), 275.1 bits, see alignment E=4.9e-86

Best Hits

KEGG orthology group: K10680, N-ethylmaleimide reductase [EC: 1.-.-.-] (inferred from 100% identity to bpy:Bphyt_5482)

Predicted SEED Role

"NADH:flavin oxidoreductases, Old Yellow Enzyme family"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TGR6 at UniProt or InterPro

Protein Sequence (368 amino acids)

>BPHYT_RS27240 N-ethylmaleimide reductase (Burkholderia phytofirmans PsJN)
MSKLFEPVKVGPYNLRHRAVMAPLTRSRASQPGNIPSQLNACYYAQRASAALIISEATQV
SMQGQGYAWTPGIHSREQVEGWRLVVNAVHEAGGLMFMQLWHVGRISHPSLQPDEMLPVA
PSAVEPKAEAFIENERGEGVVAPCITPRALQVEEMPYLVRQYFRGARNAKAAGMDGVEVH
AANGYLLDQFLLSGTNRRTDSYGGSRERRAKLLLEVIETVCDVWGPEKVGVRLSPLGTFN
DMHDDDPEATFSYVLEKLNQYGLAYLHIVNPALAATSEDVAFTERAKRMSEMVRRTYRGV
LMVAGGFDGGSAERWLEEGNADLIAFGRLFIANPDLPERLRLHAPLNSPDPSTFYGGGER
GYTDYPSL