Protein Info for BPHYT_RS27220 in Burkholderia phytofirmans PsJN

Annotation: galactarate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08666: SAF
  • amino acids 13 to 83 (71 residues), 32.5 bits, E=1.6e-11
• PF04295: GD_AH_second
  • amino acids 113 to 254 (142 residues), 155.7 bits, E=1.2e-49
• PF20629: GD_AH_C
  • amino acids 265 to 505 (241 residues), 340.5 bits, E=7.4e-106

Best Hits

KEGG orthology group: K01685, altronate hydrolase [EC: 4.2.1.7] (inferred from 100% identity to bpy:Bphyt_5478)

Predicted SEED Role

"Altronate dehydratase (EC 4.2.1.7)" (EC 4.2.1.7)

MetaCyc Pathways

• D-galacturonate degradation I (4/5 steps found)
• superpathway of hexuronide and hexuronate degradation (7/10 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (20/31 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.7

Use Curated BLAST to search for 4.2.1.7

Sequence Analysis Tools

See B2TGQ1 at UniProt or InterPro

Protein Sequence (508 amino acids)

>BPHYT_RS27220 galactarate dehydratase (Burkholderia phytofirmans PsJN)
MTDQTIVILHNEDNVAVALRALEAGTPVEAGGQRVVVRGSIAAGHKLAIRRIARGSAVTK
YRQTIGIALADVEPGEHVHVHNVGMPEHHPHRPTHSDAVRSLAPVDRPGAFQGFVRADGQ
VGTRNYIGVIASVNCSASVCHAIADAFKGDAMDPFSTVDGVVAITHQSGCGMSATGDGMA
LLRRTLTGYACNPNFAGVLLVGLGCEVNQVSGLTRSLEAGSPALVETLVIQDEGGVRETV
ARGIAIVREMLSAASRIRRIEVPAARLKVGLQCGGSDGYSGITANPALGAAVDLLVRHGG
TAILSETPEIYGAEHLLTARAVSSDVAGRLLDKLRWWERYAAEAGGDMNNNPSPGNKAGG
ITTILEKSLGAVSKGGSTALNAVYEYAERASESGLVFMDTPGYDPVSATGQIAGGANLVC
FTTGRGSVFGSKPVPTIKLATTTGLFERMRADMDFNCGQIVDGSLTVEQAGAQVFQLMLD
IASGTKTHSEQNGMGDREFVPWLRGAIM