Protein Info for BPHYT_RS26895 in Burkholderia phytofirmans PsJN

Annotation: nicotinate phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 PF18127: NAMPT_N" amino acids 19 to 110 (92 residues), 108.1 bits, see alignment E=2.4e-35 PF04095: NAPRTase" amino acids 182 to 420 (239 residues), 191.9 bits, see alignment E=1.5e-60

Best Hits

Swiss-Prot: 45% identical to NAMPT_PIG: Nicotinamide phosphoribosyltransferase (NAMPT) from Sus scrofa

KEGG orthology group: K00763, nicotinate phosphoribosyltransferase [EC: 2.4.2.11] (inferred from 100% identity to bpy:Bphyt_5413)

MetaCyc: 63% identical to nicotinamide phosphoribosyltransferase (Synechocystis sp. PCC 6803)
Nicotinamide phosphoribosyltransferase. [EC: 2.4.2.12]

Predicted SEED Role

"Nicotinamide phosphoribosyltransferase (EC 2.4.2.12)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 2.4.2.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.11

Use Curated BLAST to search for 2.4.2.11 or 2.4.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TBX3 at UniProt or InterPro

Protein Sequence (475 amino acids)

>BPHYT_RS26895 nicotinate phosphoribosyltransferase (Burkholderia phytofirmans PsJN)
MQNESSGPDDIASILSNPILNTDSYKASHYLQYPPEASAMFSYIESRGGRYDRTLFFGLQ
MLIKEYLCRPITPAMIEQAKAFFTAHGEPFNEAGWRYIVEHHGGYLPVRIRAVPEGSVVP
THNVLVTVECDDPQVFWLASYLETMLLRVWYPITVATQSWHLRETIRGYLLKSSDDLAQL
PFKLHDFGARGVSSAESAAIGGAAHLVSFMGSDTVLGVVAANHYYNETMAAFSVPAAEHS
TITSWGRERETDAFRNMIARFGKPGAIVSVVSDSYDLFAALNAWGTELKQAVLDSGGTLV
IRPDSGDPQTIVLQTLRALEASFGSVVNGKGRRVLNNVRVIQGDGVNPDSIEATLATMDQ
AGFATDNIVFGMGGALLQQINRDTQRFAMKCSAIRLGDEWHDVLKDPVTDAGKRSMKGRL
TLLKNHHTGEYRTTTLPVAVEGDWEEALVTVFDSGKLLVDMSLAEIRSRAHAHEG