Protein Info for BPHYT_RS26655 in Burkholderia phytofirmans PsJN

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 4 to 309 (306 residues), 398.4 bits, E=2.9e-123
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 8 to 753 (746 residues), 1074.9 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 426 to 748 (323 residues), 509.7 bits, E=3.7e-157

Best Hits

Swiss-Prot: 100% identical to METE_PARPJ: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 100% identity to bpy:Bphyt_5364)

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Sequence Analysis Tools

See B2TBS1 at UniProt or InterPro

Protein Sequence (764 amino acids)

>BPHYT_RS26655 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (Burkholderia phytofirmans PsJN)
MARTHIPGFPRIGAQRELKFAQESFWRGESDEQYLLGVARELRARHWQLQQDAKLDFVTV
GDFAYYDQMLNLSALLGALPQRFGFDAKTLSLARYYELARGNAAQPAMEMTKWFDTNYHY
LVPELGPQTTFDGGVEWLFDEIDEALALNLPVKPVLIGPITYLWLSKSHVAGFDRLSLLP
KLVIRYSRLLEKLKQRGIEWVQLDEPALCVDLPVEWLDAFSAAYDVLGTSGVKVLLATYF
ESAAEHAPRVAVLPVAGVHLDLVRAPQQLDAWRAALPKHAVLSAGVIDGRNIWRADLGEI
FESLQALHAEFGERLWVSSSCSLLHVPVSLDAEQKLDADLKSWLAFATEKLGEVATLALA
LRDPAAAEATLAAADRALDARRHSSTVVNALVQKRVAAVSSAMADRQSPFAERNRLQREA
LGLPLLPTTTIGSFPQTPAIRQARAAYKRGELRALDYLQRMRAEIEIAVRKQEELGLDVL
VHGEAERNDMVEYFGEQLWGYAFTENGWVQSYGSRCVKPPIIYGDVYRPEPMTVETTRYA
QSLTQRLMKGMLTGPVTMLQWSFVRDDQPRSTTALQLALAIRDEVVDLEKAGIRIIQIDE
PAFREGLPLRRGDWAAYLEWATRVFRISAAGVADQTQIHTHMCYSEFNDILPSIAAMDAD
VITIETSRSAMELLDGFGAFAYPNEIGPGVYDIHSPRVPDAQAMQRLLERACEVIPAERL
WVNPDCGLKTRGWPETEAALTNMVRAAKALRAKLAAKQPDEVTA