Protein Info for BPHYT_RS26530 in Burkholderia phytofirmans PsJN

Annotation: amino acid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 409 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF01266: DAO" amino acids 4 to 391 (388 residues), 187.4 bits, see alignment E=8.6e-59 PF13454: NAD_binding_9" amino acids 5 to 89 (85 residues), 26.5 bits, see alignment E=8.7e-10 PF13450: NAD_binding_8" amino acids 6 to 33 (28 residues), 22 bits, see alignment (E = 2.4e-08)

Best Hits

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 100% identity to bpy:Bphyt_5338)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TBP5 at UniProt or InterPro

Protein Sequence (409 amino acids)

>BPHYT_RS26530 amino acid dehydrogenase (Burkholderia phytofirmans PsJN)
MPEIAIIGAGFIGLASAVALMRDGHRVTLFDPAGVGQGASFGNAGTFAHYACIPVNNPSV
FRDLPRFLLSNQSPFRLRWGYLPHLTPWLVRFMMSSLPQRYETSAGALAALLDCAQDGYA
PLLANAELARFVRPRECLYLYSNAASFDAARPALDLRHKLGVAFEVLDGAAIRALEPALA
PIFERGVLFSNSWHFSDPQGFLQTLYEQLAAQGLKLERSTVDAVQPGTDSASLTVGGMTR
RFDHVVVATGARSAQFASQCGDSVPLDTERGYHVRFPGAQQLVSRPVGWAERGFYMTPMS
DGLRVAGTVELAGFGDTRNRSLLDLLTFSSKRALPALDTPDSDWLGFRPTLPDGVPVLAR
SRASARVIYAFGHQHLGLTLAGVSGRIVADLIAQRAPPLDLAPYAATRF