Protein Info for BPHYT_RS26480 in Burkholderia phytofirmans PsJN

Annotation: peptidase M1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF17900: Peptidase_M1_N" amino acids 79 to 284 (206 residues), 86.1 bits, see alignment E=3.4e-28 PF01433: Peptidase_M1" amino acids 326 to 543 (218 residues), 152 bits, see alignment E=1.9e-48

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 100% identity to bpy:Bphyt_5329)

Predicted SEED Role

"Puromycin-sensitive aminopeptidase (EC 3.4.11.-)" (EC 3.4.11.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.-, 3.4.11.2

Use Curated BLAST to search for 3.4.11.- or 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TBN6 at UniProt or InterPro

Protein Sequence (727 amino acids)

>BPHYT_RS26480 peptidase M1 (Burkholderia phytofirmans PsJN)
MRINFRLQMQCLALAGIAILAGCGGSDGSSSLTSKSAQAPAVASGASEVALAPPAAPVVA
DTSINKTVPPVELPDTVTPINYKLWFRPNPALSSFDGRADVQIKVQKAVNAITIAGHRVK
FTNGTITLQPGNIALIATPQDDGDFYQLRPVSGQIAAGNYSLHMEWSGIINFKTYDDPVA
KTGGSCGDDPYPGCSAAEGVFRVDLKSTDGTTSGAILTQGESNLARQWFPGWDEPAFRPT
YEVSAEVPQNWNVVSNAAEKPAVNVDSGYKLVSFEKTPPMPSYLLFFGGGQFDILEDDFT
SPLPDGKGLHLRIFTPPGMRDWAKPAMQQTKQALDYYYRYTGIPLPLTKFDTIAANDAFK
EQKDLNFGGMENWGAILEFADDILPAPGTTMSDYGVTVLTHEAAHQWFGDLVTLDWWDDV
WLNESFATFFENKTKVRFFPDRFSWVDDVKNKYAVINADLKSTAFPVQPNFNGWASNDFV
LSASAFTYDKGGHVLKMLENYLGEETMRKGLQSYLADYSLGNVTPRRLWDELSKASGQPM
VAIGDSFVRQTGVPLISLDTQCDLTTNQTVVTLKQSPFPNQNQYPGTQWTIPLTLAYGDG
LTSRKTLAMKDTQTQVRLNGCSAVLADPSGLDYYVTNYSNSAWSQLLAQGNALKDPVLLT
SLQLEAKLLVNNGLADPSRVTSIGSLSPAATPLARQLLMTAPTTQSQRPTIRYQGKFKLK
PQAQQGQ