Protein Info for BPHYT_RS26375 in Burkholderia phytofirmans PsJN

Annotation: ATPase AAA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07728: AAA_5
  • amino acids 162 to 281 (120 residues), 73.4 bits, E=1.9e-24
• PF08406: CbbQ_C
  • amino acids 328 to 397 (70 residues), 30 bits, E=5.1e-11

Best Hits

KEGG orthology group: K09882, cobaltochelatase CobS [EC: 6.6.1.2] (inferred from 100% identity to bpy:Bphyt_5308)

Predicted SEED Role

"Aerobic cobaltochelatase CobS subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (30/33 steps found)
• cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (12/13 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

See B2TBL5 at UniProt or InterPro

Protein Sequence (409 amino acids)

>BPHYT_RS26375 ATPase AAA (Burkholderia phytofirmans PsJN)
MNAPELTEDENLGLYQRATENGAEWWRVSVADRPENYRLEHGVDNGGGLGAVDIDLVVDA
ENLRAKLKKWRREGFAMDTANAASDQSPAGRVAFMPELQRAAAQASAAKHRQPEGTGETV
RIGHVEVPCGVGNPLVPRINPAYLFSERFNDIVEDIVENRRVMLIGHTGAGKTSLIEQVA
ARSRHGVLRSNMNGQTTVGDFVGFWTVKGGETLWVDGVLPTAMREGLWLIVDEIDFAEPS
ILAALTAVLEPHGRLVLKEKGNEIVAPHPAFRLFATANAVGAMSQFRHLYQGANLMNEAF
LDRWRVYLLDYLTPAEEADVLIATLAPHMTRALATTLAAIAADCRAAFAREDLSSAFSTR
RLLDWAELMLRTGDPERAAGPAIYAKVSPEDAALIRGIVRHHIAPAPQA