Protein Info for BPHYT_RS26220 in Burkholderia phytofirmans PsJN

Annotation: 6-phosphogluconate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 345 TIGR00872: 6-phosphogluconate dehydrogenase (decarboxylating)" amino acids 1 to 337 (337 residues), 417.3 bits, see alignment E=1.7e-129 PF03446: NAD_binding_2" amino acids 2 to 152 (151 residues), 131 bits, see alignment E=6.6e-42 PF03807: F420_oxidored" amino acids 3 to 92 (90 residues), 24.8 bits, see alignment E=4.1e-09 PF00393: 6PGD" amino acids 184 to 212 (29 residues), 36.4 bits, see alignment (E = 6.1e-13) amino acids 233 to 332 (100 residues), 33.9 bits, see alignment E=3.5e-12

Best Hits

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 100% identity to bpy:Bphyt_5277)

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.44

Use Curated BLAST to search for 1.1.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TDC5 at UniProt or InterPro

Protein Sequence (345 amino acids)

>BPHYT_RS26220 6-phosphogluconate dehydrogenase (Burkholderia phytofirmans PsJN)
MQLGMIGLGRMGADMVRRLTKGGQQCIAYDVQPAAVEKLKQDGVPGAASLEDLVAQLDKP
RAVWLMVPAAVVDMTLEKLVPLLEPGDIVIDGGNSYYHDDIRRGTELAERKLHYVDVGTS
GGVAGRERGYCLMIGGEPEVVKRLEPIFATLAPGAGSEPATPGRVAGSSTADQGFLHCGP
QGAGHFVKMVHNGIEYGMMAAYAEGLNILHHADAGKHAREADAETSPLRRPELYQYDLNL
ADVTEVWRRGSVISSWLLDLIAGSLVSDVDLKNYAGRVSDSGEGRWTVAAAIDEGVPTPV
LSAALFARFSSRGEADFANRVLSAMRNDFGGHAEKAATPSDKPKS