Protein Info for BPHYT_RS26030 in Burkholderia phytofirmans PsJN

Annotation: cysteine hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00857: Isochorismatase
  • amino acids 23 to 211 (189 residues), 142.5 bits, E=7.9e-46

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5238)

MetaCyc: 48% identical to biuret amidohydrolase subunit (Rhizobium leguminosarum bv. viciae 3841)
Biuret amidohydrolase. [EC: 3.5.1.84]

Predicted SEED Role

"Isochorismatase (EC 3.3.2.1)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. (EC 3.3.2.1)

MetaCyc Pathways

• cyanuric acid degradation II (4/5 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• 2,3-dihydroxybenzoate biosynthesis (1/3 steps found)
• superpathway of atrazine degradation (4/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• vibriobactin biosynthesis (1/9 steps found)
• bacillibactin biosynthesis (3/12 steps found)

KEGG Metabolic Maps

• Atrazine degradation
• Biosynthesis of phenylpropanoids
• Biosynthesis of siderophore group nonribosomal peptides

Isozymes

Compare fitness of predicted isozymes for: 3.3.2.1

Use Curated BLAST to search for 3.3.2.1 or 3.5.1.84

Sequence Analysis Tools

See B2TD86 at UniProt or InterPro

Protein Sequence (231 amino acids)

>BPHYT_RS26030 cysteine hydrolase (Burkholderia phytofirmans PsJN)
MTSTNTLTIDAQPGPFSFDSAKTALVVIDMQRDFIEPGGFGESLGNDVSLLADIVPTVAA
LLAFARKHHWFVVHTRESHAPDLSDCPPAKRLRGAPNARIGDAGPMGRILIRGEPGNAIV
EPLAPLAGELVIDKPGKGAFHATRLGEELAMRGVTHLVFAGVTTEVCVQTSMREANDRGY
DSLLIEDATASYFPAFKQATLEMVRSQGGIVGWTAPFASLIKTDETTQAWK