Protein Info for BPHYT_RS25970 in Burkholderia phytofirmans PsJN

Annotation: urea amidolyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02713: allophanate hydrolase
  • amino acids 45 to 468 (424 residues), 582.2 bits, E=5.3e-179
• PF01425: Amidase
  • amino acids 68 to 452 (385 residues), 287.9 bits, E=8.6e-90

Best Hits

KEGG orthology group: K01457, allophanate hydrolase [EC: 3.5.1.54] (inferred from 100% identity to bpy:Bphyt_5227)

Predicted SEED Role

"Allophanate hydrolase (EC 3.5.1.54)" in subsystem Urea decomposition (EC 3.5.1.54)

MetaCyc Pathways

• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• cyanate degradation (3/3 steps found)
• urea degradation I (3/3 steps found)
• superpathway of allantoin degradation in yeast (5/6 steps found)
• cyanuric acid degradation II (4/5 steps found)
• uracil degradation III (3/5 steps found)
• cyanuric acid degradation I (2/5 steps found)
• superpathway of atrazine degradation (4/8 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.54

Use Curated BLAST to search for 3.5.1.54

Sequence Analysis Tools

See B2TD09 at UniProt or InterPro

Protein Sequence (484 amino acids)

>BPHYT_RS25970 urea amidolyase (Burkholderia phytofirmans PsJN)
MKSHDDLFQSMSIEALRARYRAGSLTPAQLVEAVAARTSGDDPHRAWIRPLSRNEMIRYV
DALEGRDIDTLPLYGVPFAIKDNIDLAGIPTTAACPAYAYTPEASAPVVARLIAAGAIPI
GKTNLDQFATGLSGQRSPHGACRNALDPRYASGGSSSGSAVSVALGLAAFSLGTDTAGSG
RVPAAFHGLLGLKPTKGVLSTLGVVPACKSLDCVSIFARSADDAQCVFDAARGVAPSDPY
AREFQPPTGAAQLGAQPSLRGVRFGVPRANQLEFFGDTSYAHAFDAALTRLRAAGAELVE
IDFEPFLATARLLYEGPWVAERLAAIREFADTQPDALHPVIREIIGGAARWSAADAFAAF
DRLAFLRMQAAHVWQRIDAIVTPTSATTATVDELEADPIRVNSRFGYYTNFVNLLDLAAL
AVPAGVCTVGRHAGLPFGVTFVARAHEDRVLLDLARAWLDETTAASRSAYDATAVRLGES
HGRH