Protein Info for BPHYT_RS25630 in Burkholderia phytofirmans PsJN
Annotation: glutathione S-transferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 71% identical to YGHU_ECOLI: Disulfide-bond oxidoreductase YghU (yghU) from Escherichia coli (strain K12)
KEGG orthology group: K11209, GST-like protein (inferred from 100% identity to bpy:Bphyt_5157)MetaCyc: 71% identical to disulfide reductase / organic hydroperoxide reductase (Escherichia coli K-12 substr. MG1655)
RXN0-6256
Predicted SEED Role
"Glutathione S-transferase (EC 2.5.1.18)" in subsystem Glutathione: Non-redox reactions (EC 2.5.1.18)
MetaCyc Pathways
- 4-hydroxy-2-nonenal detoxification (1/4 steps found)
- pentachlorophenol degradation (5/10 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
- gliotoxin biosynthesis (2/9 steps found)
- glutathione-mediated detoxification II (1/9 steps found)
- indole glucosinolate activation (intact plant cell) (3/12 steps found)
- camalexin biosynthesis (2/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.18
Use Curated BLAST to search for 2.5.1.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2TCU4 at UniProt or InterPro
Protein Sequence (292 amino acids)
>BPHYT_RS25630 glutathione S-transferase (Burkholderia phytofirmans PsJN) MADSHDYIPPKVWTWNKPSGGAFANINRPIAGPTHEKVLPVGRHPLQLYSLGTPNGVKVT ILLEELLAAGYAGAEYDAWLIRIGEGEQFGSGFVDINPNSKIPALLDRSGPKPVRVFESG SILLYLAEKFGAFLPKDVETRTETLNWLFWQMGSAPYLGGGFGHFYAYAPMKIEYAIDRF AMEVKRQLDVLDQRLAGNEYLAGDEYTIADMAVFPWYGGLVKGWQYGAAEFLSVQDYANV QRWADMLLARPAVRRGRMVNRTSGDLSGQLRERHDASDFETTTQDRIEGVHP