Protein Info for BPHYT_RS25590 in Burkholderia phytofirmans PsJN
Annotation: FAD-dependent pyridine nucleotide-disulfide oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K03885, NADH dehydrogenase [EC: 1.6.99.3] (inferred from 100% identity to bpy:Bphyt_5149)Predicted SEED Role
"NADH dehydrogenase (EC 1.6.99.3)" in subsystem Carboxysome or Respiratory dehydrogenases 1 (EC 1.6.99.3)
MetaCyc Pathways
- aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
- NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
- NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
- aerobic respiration I (cytochrome c) (3/4 steps found)
- NAD(P)/NADPH interconversion (3/6 steps found)
- Fe(II) oxidation (2/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.6.99.3
Use Curated BLAST to search for 1.6.99.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2TCT6 at UniProt or InterPro
Protein Sequence (696 amino acids)
>BPHYT_RS25590 FAD-dependent pyridine nucleotide-disulfide oxidoreductase (Burkholderia phytofirmans PsJN) MLRKTLKRACGFGLALRVLCMPWVLLFARIWFGQVVFVHQIMTMAEGPNDGLFSGGLHVP SGLDAALHSVVPLLLTMGLLARPVALVLLIETIADSHGALAVGAQGAKLALLSWLIVSGA GAMSLDNLLGRGVSWIPFRPIRLTRGLYVWFERYLGPLLQFVIRAGLATSLVNFVLPTGS LLQKSMLGAAATSPVHPAWCAAALALALTLGCATRFAALVIAVMVPVAGIAMSTDDRLAV LLLLLVLVVAGGGAFSVDWLLDRWTGTKSQEDDSDPEDLPHVVVVGGGFGGVAAAQGLRN ARCRVTLIDQRNHHLFQPLLYQVATAALSPAEIATPIRSLFRRQRNVQVMLGQVTGVDAV AREVHVGTLAVKFDYLVLATGARHSYFGKDSWAPFAPGLKSIEDATDVRSRLLRAFEEAE SAASETERAAWLTFVIVGGGPTGIELAGAIAELARHGMAEEYRKIDPARARVILLQSGPR ILPTFAATLSGAAERSLQALGVEVRLDTRVLGVDSEGVDIGGQRIAARTVLWAAGVAASP AAQWLNRTADPSGRLAVGADLSVPDLQRIYAIGDTASCLGWRGAVVPGLAPAAKQQGLYV AGVIAASLSGRRPPPPFRYRHFGSLATIGRQAAVAEIGKLRVWGEPAWWFWGAIHIAFLV GGRNRAIVVLDWLWAYLTYRRSTRLITGDTRDKMMD