Protein Info for BPHYT_RS25405 in Burkholderia phytofirmans PsJN

Annotation: CoA-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 43% identical to AMACR_HUMAN: Alpha-methylacyl-CoA racemase (AMACR) from Homo sapiens

KEGG orthology group: K01067, acetyl-CoA hydrolase [EC: 3.1.2.1] (inferred from 100% identity to bpy:Bphyt_5109)

MetaCyc: 48% identical to alpha-methylacyl-CoA racemase (Mycobacterium tuberculosis H37Rv)
Alpha-methylacyl-CoA racemase. [EC: 5.1.99.4]; 5.1.99.4 [EC: 5.1.99.4]; 5.1.99.4 [EC: 5.1.99.4]; 5.1.99.4 [EC: 5.1.99.4]

Predicted SEED Role

"Alpha-methylacyl-CoA racemase (EC 5.1.99.4)" (EC 5.1.99.4)

MetaCyc Pathways

cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
cholesterol degradation to androstenedione III (anaerobic) (1/22 steps found)
bile acid biosynthesis, neutral pathway (2/29 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.1, 5.1.99.4

Use Curated BLAST to search for 3.1.2.1 or 5.1.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TCP8 at UniProt or InterPro

Protein Sequence (357 amino acids)

>BPHYT_RS25405 CoA-transferase (Burkholderia phytofirmans PsJN)
MTTKRQGPLAGLKVLEIAGIGPGPFCAMLFADLGADVVVVDRIEAGPDALDLGGTQLANR
GKRSVAVDLKTVEGVETVLRLIEQSDALIEGMRPGVMERLGLGPDICLGRNSRLIYGRMT
GWGQDGPLAHAAGHDLNYIALSGALWYAGQPGEAPVTPPSLVGDVGGGAMYLAVGLLAGI
MNARATGVGQVVDAAIVDGSAHMTTLLLALQASGQMRSERGQSLLDGPHWYNTYRCAEGG
FISVGSLEPKFYRELREKLGLGADPLFDRPYDASSWPQLRERFATLFLERSRDEWCALLE
GTDACFAPVLSPDEAATHPHMHARGVYTRDDGVLQAAPAPRFSATPASRPGPIPRRG